Cryo-EM structure of a monomeric RC-LH1-PufX supercomplex with high-carotenoid content from Rhodobacter capsulatus



Bracun, laura ORCID: 0000-0001-7698-3718, Yamagata, Atsushi, Christianson, Bern, Shirouzu, Mikako and Liu, Luning ORCID: 0000-0002-8884-4819
(2023) Cryo-EM structure of a monomeric RC-LH1-PufX supercomplex with high-carotenoid content from Rhodobacter capsulatus. Structure, 31 (3). pp. 318-328.

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Abstract

xclusively monomers in which the RC is surrounded by a 15-subunit LH1 ring. Incorporation of a transmembrane polypeptide PufX leads to a large opening within the LH1 ring. Each LH1 subunit associates two carotenoids and two bacteriochlorophylls, which is similar to Rba. sphaeroides RC-LH1 but more than one carotenoid per LH1 in Rba. veldkampii RC-LH1 monomer. Collectively, the unique Rba. capsulatus RC-LH1-PufX represents an intermediate structure between Rba. sphaeroides and Rba. veldkampii RC-LH1-PufX. Comparison of PufX from the three Rhodobacter species indicates the important residues involved in dimerization of RC-LH1.

Item Type: Article
Uncontrolled Keywords: Rhodobacter capsulatus, Rhodobacter sphaeroides, Carotenoids, Light-Harvesting Protein Complexes, Cryoelectron Microscopy
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User: Symplectic Admin
Date Deposited: 06 Feb 2023 08:45
Last Modified: 12 Oct 2023 15:55
DOI: 10.1016/j.str.2023.01.006
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3168187