Wang, Yingxue 
ORCID: 0000-0001-6814-0382, Zhang, Weijiao, Jefferson, Matthew, Sharma, Parul, Bone, Ben, Kipar, Anja, Coombes, Janine L ORCID: 0000-0001-5901-9951, Pearson, Timothy, Man, Angela, Zhekova, Alex et al (show 8 more authors)
(2020)
The WD and linker domains of ATG16L1 required for non-canonical autophagy limit lethal respiratory infection by influenza A virus at epithelial surfaces.
2020.01.15.907873-.
Abstract
<jats:title>Summary</jats:title><jats:p>Respiratory viruses such as influenza A virus (IAV) and SARS-CoV-2 (Covid-19) cause pandemic infections where cytokine storm syndrome, lung inflammation and pneumonia lead to high mortality. Given the high social and economic cost of these viruses, there is an urgent need for a comprehensive understanding of how the airways defend against virus infection. Viruses entering cells by endocytosis are killed when delivered to lysosomes for degradation. Lysosome delivery is facilitated by non-canonical autophagy pathways that conjugate LC3 to endo-lysosome compartments to enhance lysosome fusion. Here we use mice lacking the WD and linker domains of ATG16L1 to demonstrate that non-canonical autophagy protects mice from lethal IAV infection of the airways. Mice with systemic loss of non-canonical autophagy are exquisitely sensitive to low-pathogenicity murine-adapted IAV where extensive viral replication throughout the lungs, coupled with cytokine amplification mediated by plasmacytoid dendritic cells, leads to fulminant pneumonia, lung inflammation and high mortality. IAV infection was controlled within epithelial barriers where non-canonical autophagy slowed fusion of IAV with endosomes and reduced activation of interferon signalling. This was consistent with conditional mouse models and <jats:italic>ex vivo</jats:italic> analysis showing that protection against IAV infection of lung was independent of phagocytes and other leukocytes. This establishes non-canonical autophagy pathways in airway epithelial cells as a novel innate defence mechanism that can restrict IAV infection and lethal inflammation at respiratory surfaces.</jats:p>
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Lung, Pneumonia, Prevention, Infectious Diseases, Pneumonia & Influenza, Influenza, Biodefense, Emerging Infectious Diseases, Vaccine Related, 2.1 Biological and endogenous factors, 2 Aetiology, Respiratory, Infection, 3 Good Health and Well Being |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 15 Feb 2023 09:26 |
| Last Modified: | 15 Mar 2024 03:14 |
| DOI: | 10.1101/2020.01.15.907873 |
| Open Access URL: | https://www.embopress.org/doi/full/10.15252/embj.2... |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3168420 |
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