Shams, Sahand, Lima, Cassio 
ORCID: 0000-0002-9062-6298, Xu, Yun ORCID: 0000-0003-3228-5111, Ahmed, Shwan, Goodacre, Royston ORCID: 0000-0003-2230-645X and Muhamadali, Howbeer
(2023)
Optical photothermal infrared spectroscopy: A novel solution for rapid identification of antimicrobial resistance at the single-cell level <i>via</i> deuterium isotope labeling.
FRONTIERS IN MICROBIOLOGY, 14.
1077106-.
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Abstract
The rise and extensive spread of antimicrobial resistance (AMR) has become a growing concern, and a threat to the environment and human health globally. The majority of current AMR identification methods used in clinical setting are based on traditional microbiology culture-dependent techniques which are time-consuming or expensive to be implemented, thus appropriate antibiotic stewardship is provided retrospectively which means the first line of treatment is to hope that a broad-spectrum antibiotic works. Hence, culture-independent and single-cell technologies are needed to allow for rapid detection and identification of antimicrobial-resistant bacteria and to support a more targeted and effective antibiotic therapy preventing further development and spread of AMR. In this study, for the first time, a non-destructive phenotyping method of optical photothermal infrared (O-PTIR) spectroscopy, coupled with deuterium isotope probing (DIP) and multivariate statistical analysis was employed as a metabolic fingerprinting approach to detect AMR in Uropathogenic <i>Escherichia coli</i> (UPEC) at both single-cell and population levels. Principal component-discriminant function analysis (PC-DFA) of FT-IR and O-PTIR spectral data showed clear clustering patterns as a result of distinctive spectral shifts (C-D signature peaks) originating from deuterium incorporation into bacterial cells, allowing for rapid detection and classification of sensitive and resistant isolates at the single-cell level. Furthermore, the single-frequency images obtained using the C-D signature peak at 2,163 cm<sup>-1</sup> clearly displayed the reduced ability of the trimethoprim-sensitive strain for incorporating deuterium when exposed to this antibiotic, compared to the untreated condition. Hence, the results of this study indicated that O-PTIR can be employed as an efficient tool for the rapid detection of AMR at the single-cell level.
| Item Type: | Article |
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| Uncontrolled Keywords: | antimicrobial resistance, single-cell, microbiology, infrared spectroscopy, stable-isotope probing, Uropathogenic Escherichia coli |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 03 Mar 2023 15:52 |
| Last Modified: | 17 Oct 2023 21:47 |
| DOI: | 10.3389/fmicb.2023.1077106 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3168744 |
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