Bonfim Damasceno Oliveira, Samara Rodrigues, Franco, Alvaro Xavier, Quaresma, Marielle Pires, Morais de Carvalho, Cecilia Mendes, Jacome Marques, Fabricia da Cunha, Pantoja, Patricia da Silva, Mendonca, Vanessa Azevedo, da Silva Osterne, Vinicius Jose, Almeida Correia, Jorge Luis, Sampaio Assreuy, Ana Maria et al (show 5 more authors)
(2022)
Anti-inflammatory and anti-necrotic effects of lectins from <i>Canavalia ensiformis</i> and <i>Canavalia brasiliensis</i> in experimental acute pancreatitis.
GLYCOCONJUGATE JOURNAL, 39 (5).
pp. 599-608.
Abstract
Lectins isolated from Canavalia ensiformis (ConA) and Canavalia brasiliensis (ConBr) are promising molecules to prevent cell death. Acute pancreatitis, characterized by acinar cell necrosis and inflammation, presents significant morbidity and mortality. This study has investigated the effects of ConA and ConBr in experimental acute pancreatitis and pancreatic acinar cell death induced by bile acid. Pancreatitis was induced by retrograde pancreatic ductal injection of 3% sodium taurocholate (Na-TC) in male Swiss mice. ConA or ConBr (0.1, 1 or 10 mg/kg) were intravenously applied to mice 1 h and 12 h after induction. After 24 h, the severity of pancreatitis was evaluated by serum amylase and lipase, histopathological changes and myeloperoxidase assay. Pancreatic acinar cells were incubated with ConA (200 µg/ml) or ConBr (200 µg/ml) and taurolithocholic acid 3-sulfate (TLCS; 500 µM). Necrosis and changes in mitochondrial membrane potential (ΔѰm) were detected by fluorescence confocal microscopy. Treatment (post-insult) with ConA and ConBr decreased pancreatic damage caused by retrograde injection of Na-TC in mice, reducing pancreatic neutrophil infiltration, edema and necrosis. In addition, ConA and ConBr decreased pancreatic acinar cell necrosis and depolarization of ΔѰm caused by TLCS. The inhibition of necrosis was prevented by the lectin domain blockade. In conclusion, ConA and ConBr markedly inhibited in vitro and in vivo damage, effects partly dependent on the interaction with mannose residues on acinar cells. These data support the potential application of these proteins for treatment of acute pancreatitis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Lectins, Anti-inflammatory, Anti-necrotic, Acute Pancreatitis |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 24 Mar 2023 15:34 |
| Last Modified: | 19 Oct 2023 08:50 |
| DOI: | 10.1007/s10719-022-10048-w |
| Open Access URL: | https://assets.researchsquare.com/files/rs-172690/... |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3169242 |
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