Fraser, Alice J, Greenland-Bews, Caitlin, Kelly, Daniel, Williams, Christopher T, Body, Richard, Adams, Emily R, Atienzar, Ana Cubes, Edwards, Thomas and Allen, David J
(2023)
A high-resolution melt curve toolkit to identify lineage-defining SARS-CoV-2 mutations.
SCIENTIFIC REPORTS, 13 (1).
3887-.
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Abstract
The emergence of severe acute respiratory syndrome 2 (SARS-CoV-2) variants of concern (VOCs), with mutations linked to increased transmissibility, vaccine escape and virulence, has necessitated the widespread genomic surveillance of SARS-CoV-2. This has placed a strain on global sequencing capacity, especially in areas lacking the resources for large scale sequencing activities. Here we have developed three separate multiplex high-resolution melting assays to enable the identification of Alpha, Beta, Delta and Omicron VOCs. The assays were evaluated against whole genome sequencing on upper-respiratory swab samples collected during the Alpha, Delta and Omicron [BA.1] waves of the UK pandemic. The sensitivities of the eight individual primer sets were all 100%, and specificity ranged from 94.6 to 100%. The multiplex HRM assays have potential as a tool for high throughput surveillance of SARS-CoV-2 VOCs, particularly in areas with limited genomics facilities.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | LSTM Diagnostics Group, CONDOR Steering Group, Humans, Biological Assay, Genomics, Mutation, COVID-19, SARS-CoV-2 |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 05 Apr 2023 10:12 |
| Last Modified: | 05 Oct 2023 12:54 |
| DOI: | 10.1038/s41598-023-30754-1 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3169466 |
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