Advances in high throughput LC/MS based metabolomics: A review

Plumb, Robert S ORCID: 0000-0002-1380-9285, Gethings, Lee A, Rainville, Paul D, Isaac, Giorgis, Trengove, Robert, King, Adam M and Wilson, Ian D ORCID: 0000-0002-8558-7394 (2023) Advances in high throughput LC/MS based metabolomics: A review. TrAC Trends in Analytical Chemistry, 160. p. 116954.

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Official URL: http://dx.doi.org/10.1016/j.trac.2023.116954

Abstract

Properly implemented, metabolic and lipidomic profiling can provide a deeper understanding of mammalian, plant and bacterial biology. These omics-tools have developed and matured over the last 40-years and are now being deployed to provide valuable information in epidemiological studies, drug toxicology and pharmacology, disease biology and progression and patient stratification. LC/MS has become the technology of choice for both metabolic and lipid profiling, due to its speed, sensitivity and structural elucidation capabilities. In the preceding two decades there have been many technological and methodological advances in LC/MS that have facilitated the evolution of the technology into a rugged, reliable, and easily deployed tool. These advances include, but are not limited to, improvements in chromatography (phases, columns, and delivery system), instruments for mass spectrometry, optimization of sample preparation, the introduction of ion mobility, data analysis tools, metabolite databases, harmonized protocols, and the more widespread use of quality control methods and reference standards/matrices. Here, recent developments and advances in high throughput liquid chromatography/high resolution mass spectrometry for metabolic phenotyping are described. These advances which may provide improved feature detection, increased laboratory efficiency and data quality, as well as “biomarker” identification, are discussed in relation to their potential application to the analysis of large clinical studies, or biobank collections.

Item Type:	Article
Uncontrolled Keywords:	3 Good Health and Well Being
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User:	Symplectic Admin
Date Deposited:	17 Apr 2023 09:48
Last Modified:	17 Mar 2024 16:14
DOI:	10.1016/j.trac.2023.116954
Open Access URL:	https://doi.org/10.1016/j.trac.2023.116954
Related URLs:	Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3169596