Freeman, David ME, Ming, Damien K, Wilson, Richard 
ORCID: 0000-0002-3275-6932, Herzog, Peter L, Schulz, Christopher, Felice, Alfons KG, Chen, Yu-Chih, O'Hare, Danny, Holmes, Alison H ORCID: 0000-0001-5554-5743 and Cass, Anthony EG
(2023)
Continuous Measurement of Lactate Concentration in Human Subjects through Direct Electron Transfer from Enzymes to Microneedle Electrodes.
ACS SENSORS, 8 (4).
pp. 1639-1647.
Abstract
Microneedle lactate sensors may be used to continuously measure lactate concentration in the interstitial fluid in a minimally invasive and pain-free manner. First- and second-generation enzymatic sensors produce a redox-active product that is electrochemically sensed at the electrode surface. Direct electron transfer enzymes produce electrons directly as the product of enzymatic action; in this study, a direct electron transfer enzyme specific to lactate has been immobilized onto a microneedle surface to create lactate-sensing devices that function at low applied voltages (0.2 V). These devices have been validated in a small study of human volunteers; lactate concentrations were raised and lowered through physical exercise and subsequent rest. Lactazyme microneedle devices show good agreement with concurrently obtained and analyzed serum lactate levels.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | biosensor, continuous monitoring, direct electron transfer enzyme, in-human study, lactate, microneedles |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 20 Apr 2023 08:23 |
| Last Modified: | 01 May 2023 02:51 |
| DOI: | 10.1021/acssensors.2c02780 |
| Open Access URL: | https://doi.org/10.1021/acssensors.2c02780 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3169788 |
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