Nyazika, Tinashe K, Sibale, Lusako, Phiri, Joseph, De Ste Croix, Megan, Jasiunaite, Zydrune, Mkandawire, Christopher, Malamba, Rose, Kankwatira, Anstead, Manduwa, Miriam, Ferreira, Daniela M et al (show 4 more authors)
(2022)
Intracellular survival of <i>Streptococcus pneumoniae</i> in human alveolar macrophages is augmented with HIV infection.
FRONTIERS IN IMMUNOLOGY, 13.
992659-.
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Intracellular survival of iStreptococcus pneumoniaei in human alveolar macrophages is augmented with HIV infection.pdf - Open Access published version Download (6MB) | Preview |
Abstract
People Living with HIV (PLHIV) are at an increased risk of pneumococcal pneumonia than HIV-uninfected adults, but the reasons for this are still not well understood. We investigated whether alveolar macrophages (AM) mediated control of pneumococcal infection is impaired in PLHIV compared to HIV-uninfected adults. We assessed anti-bactericidal activity against <i>Streptococcus pneumoniae</i> of primary human AM obtained from PLHIV and HIV-uninfected adults. We found that pneumococcus survived intracellularly in AMs at least 24 hours post <i>ex vivo</i> infection, and this was more frequent in PLHIV than HIV-uninfected adults. Corroborating these findings, <i>in vivo</i> evidence showed that PLHIV had a higher propensity for harboring <i>S. pneumoniae</i> within their AMs than HIV-uninfected adults. Moreover, bacterial intracellular survival in AMs was associated with extracellular propagation of pneumococcal infection. Our data suggest that failure of AMs to eliminate <i>S. pneumoniae</i> intracellularly could contribute to the increased risk of pneumococcal pneumonia in PLHIV.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Streptococcus pneumoniae, alveolar macrophages, HIV, intracellular killing, lung |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 21 Apr 2023 13:29 |
| Last Modified: | 18 Oct 2023 10:18 |
| DOI: | 10.3389/fimmu.2022.992659 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3169850 |
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