Mewamba, Estelle Mezajou, Noyes, Harry 
ORCID: 0000-0002-0656-200X, Tiofack, Arnol Auvaker Zebaze, Kamga, Rolin Mitterran Ndefo, Kamdem, Cyrille Nguemnang, Mengoue, Loic Edmond Tekeu, Ofon, Elvis, Ngassam, Romuald Isaka Kamwa, Nyangiri, Oscar, Bucheton, Bruno et al (show 5 more authors)
(2023)
Association between polymorphisms of IL4, IL13, IL10, STAT6 and IFNG genes, cytokines and immunoglobulin E levels with high burden of Schistosoma mansoni in children from schistosomiasis endemic areas of Cameroon.
INFECTION GENETICS AND EVOLUTION, 111.
105416-.
Abstract
Eliminating schistosomiasis as a public health problem by 2030 requires a better understanding of the disease transmission, especially the asymmetric distribution of worm burden in individuals living and sharing the same environment. It is in this light that this study was designed to identify human genetic determinants associated with high burden of S. mansoni and also with the plasma concentrations of IgE and four cytokines in children from two schistosomiasis endemic areas of Cameroon. In school-aged children of schistosomiasis endemic areas of Makenene and Nom-Kandi of Cameroon, S. mansoni infections and their infection intensities were evaluated in urine and stool samples using respectively the Point-of-care Circulating Cathodic Antigen test (POC-CCA) and the Kato Katz (KK) test. Thereafter, blood samples were collected in children harbouring high burden of schistosome infections as well as in their parents and siblings. DNA extracts and plasma were obtained from blood. Polymorphisms at 14 loci of five genes were assessed using PCR-restriction fragment length polymorphism and amplification-refractory mutation system. The ELISA test enabled to determine the plasma concentrations of IgE, IL-13, IL-10, IL-4 and IFN-γ. The prevalence of S. mansoni infections was significantly higher (P < 0.0001 for POC-CCA; P = 0.001 for KK) in Makenene (48.6% for POC-CCA and 7.9% for KK) compared to Nom-Kandi (31% for POC-CCA and 4.3% for KK). The infection intensities were also higher (P < 0.0001 for POC-CCA; P = 0.001 for KK) in children from Makenene than those from Nom-Kandi. The allele C of SNP rs3024974 of STAT6 was associated with an increased risk of bearing high burden of S. mansoni both in the additive (p = 0.009) and recessive model (p = 0.01) while the allele C of SNP rs1800871 of IL10 was protective (p = 0.0009) against high burden of S. mansoni. The alleles A of SNP rs2069739 of IL13 and G of SNP rs2243283 of IL4 were associated with an increased risk of having low plasma concentrations of IL-13 (P = 0.04) and IL-10 (P = 0.04), respectively. This study showed that host genetic polymorphisms may influence the outcome (high or low worm burden) of S. mansoni infections and also the plasma concentrations of some cytokines.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Schistosoma mansoni, Circulating cathodic antigen, Cytokines, IgE, Genetic polymorphism, Infection intensity |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 03 May 2023 12:24 |
| Last Modified: | 23 Sep 2023 15:36 |
| DOI: | 10.1016/j.meegid.2023.105416 |
| Open Access URL: | https://doi.org/10.1016/j.meegid.2023.105416 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3170110 |
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