Sampsonidis, Ioannis, Marinaki, Maria, Pesiridou, Anastasia, Gika, Helen, Theodoridis, Georgios, Siachos, Nektarios 
ORCID: 0000-0001-7670-4950, Arsenos, Georgios and Kalogiannis, Stavros
(2023)
Cost-Effective Simultaneous Determination of tau- and pi-Methylhistidine in Dairy Bovine Plasma from Large Cohort Studies Using Hydrophilic Interaction Ultra-High Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry.
Separations, 10 (2).
p. 144.
Abstract
The isomeric metabolites τ- and π-methylhistidine (formerly referred to as 3- and 1-methylhistidine) are known biomarkers for muscle protein breakdown and meat protein intake, frequently used in studies involving humans and animals. In the present study, we report the development and validation of a simple HILIC-MS/MS method for individual determination of τ-MH and π-MH in a large cohort of blood plasma samples from dairy cows. Their separate determination was achieved mainly through a mass spectrometry fragment ion study, which revealed that the two isomers exhibited distinct mass spectrometric behaviors at different collision energies. Chromatographic conditions were optimised to achieve better separation, minimizing inter-channel interference to less than 1% in both directions. A simple and effective sample clean-up method facilitated low laboratory manual workload. The analytical method was validated for the determination of τ-MH and π-MH in bovine plasma within a concentration range of 80 to 1600 μg/L and provided good linearity (>0.99 for both curves) and precision (<10%). Overall, the developed method enabled the determination of the two isomers in an efficient and economic-friendly manner suitable for large cohort bovine studies (involving hundreds to thousands of samples) mainly to provide data for statistical use.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | 1-methylhistidine, 3-methylhistidine, bovine plasma, lactating cows, muscle protein breakdown |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 04 May 2023 13:48 |
| Last Modified: | 12 Oct 2023 15:54 |
| DOI: | 10.3390/separations10020144 |
| Open Access URL: | https://doi.org/10.3390/separations10020144 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3170171 |
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