Acute rotavirus infection is associated with the induction of circulating memory CD4+ T cell subsets

Collapse authors list.
Malamba-Banda, Chikondi, Mhango, Chimwemwe, Benedicto-Matambo, Prisca, Mandolo, Jonathan J, Chinyama, End, Kumwenda, Orpha, Barnes, Kayla G, Cunliffe, Nigel A ORCID: 0000-0002-5449-4988, Iturriza-Gomara, Miren, Jambo, Kondwani C et al (show 1 more authors) (2023) Acute rotavirus infection is associated with the induction of circulating memory CD4+ T cell subsets. SCIENTIFIC REPORTS, 13 (1). 9001-.

PDF
Malamba et al 2023 Acute rotavirus infection is associated with the induction of circulating memory CD4+ T cell subsets.pdf - Open Access published version
Download (1MB) | Preview

Official URL: http://dx.doi.org/10.1038/s41598-023-35681-9

Abstract

Strong CD4+ T cell-mediated immune protection following rotavirus infection has been observed in animal models, but its relevance in humans remains unclear. Here, we characterized acute and convalescent CD4+ T cell responses in children who were hospitalized with rotavirus-positive and rotavirus-negative diarrhoea in Blantyre, Malawi. Children presenting with laboratory-confirmed rotavirus infection had higher proportions of effector and central memory T helper 2 cells during acute infection i.e., at disease presentation compared to convalescence, 28 days post-infection defined by a follow-up 28 days after acute infection. However, circulating cytokine-producing (IFN-γ and/or TNF-α) rotavirus-specific VP6-specific CD4+ T cells were rarely detectable in children with rotavirus infection at both acute and convalescent stages. Moreover, following whole blood mitogenic stimulation, the responding CD4+ T cells were predominantly non-cytokine producers of IFN-γ and/or TNF-α. Our findings demonstrate limited induction of anti-viral IFN-γ and/or TNF-α-producing CD4+ T cells in rotavirus-vaccinated Malawian children following the development of laboratory-confirmed rotavirus infection.

Item Type:	Article
Uncontrolled Keywords:	T-Lymphocyte Subsets, CD4-Positive T-Lymphocytes, Animals, Humans, Rotavirus, Rotavirus Infections, Tumor Necrosis Factor-alpha, Cytokines, Child
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences
Depositing User:	Symplectic Admin
Date Deposited:	13 Jun 2023 15:55
Last Modified:	14 Oct 2023 08:17
DOI:	10.1038/s41598-023-35681-9
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3170905