<i>Plasmodium falciparum</i>-infected erythrocyte co-culture with the monocyte cell line THP-1 does not trigger production of soluble factors reducing brain microvascular barrier function

Storm, Janet, Camarda, Grazia N, Haley, Michael JG, Brough, David, Couper, Kevin N and Craig, Alister G ORCID: 0000-0003-0914-6164 (2023) <i>Plasmodium falciparum</i>-infected erythrocyte co-culture with the monocyte cell line THP-1 does not trigger production of soluble factors reducing brain microvascular barrier function. PLOS ONE, 18 (5). e0285323-.

PDF
Plasmodium falciparum-infected erythrocyte co-culture with the monocyte cell line THP-1 does not trigger production of solub.pdf - Open Access published version
Download (1MB) | Preview

Official URL: http://dx.doi.org/10.1371/journal.pone.0285323

Abstract

Monocytes contribute to the pro-inflammatory immune response during the blood stage of a Plasmodium falciparum infection, but their precise role in malaria pathology is not clear. Besides phagocytosis, monocytes are activated by products from P. falciparum infected erythrocytes (IE) and one of the activation pathways is potentially the NLR family pyrin domain containing 3 (NLRP3) inflammasome, a multi-protein complex that leads to the production of interleukin (IL)-1β. In cerebral malaria cases, monocytes accumulate at IE sequestration sites in the brain microvascular and the locally produced IL-1β, or other secreted molecules, could contribute to leakage of the blood-brain barrier. To study the activation of monocytes by IE within the brain microvasculature in an in vitro model, we co-cultured IT4var14 IE and the monocyte cell line THP-1 for 24 hours and determined whether generated soluble molecules affect barrier function of human brain microvascular endothelial cells, measured by real time trans-endothelial electrical resistance. The medium produced after co-culture did not affect endothelial barrier function and similarly no effect was measured after inducing oxidative stress by adding xanthine oxidase to the co-culture. While IL-1β does decrease barrier function, barely any IL-1β was produced in the co- cultures, indicative of a lack of or incomplete THP-1 activation by IE in this co-culture model.

Item Type:	Article
Uncontrolled Keywords:	Brain, Erythrocytes, Monocytes, Cell Line, Endothelial Cells, Humans, Plasmodium falciparum, Malaria, Falciparum, Coculture Techniques, Interleukin-1beta, Inflammasomes, NLR Family, Pyrin Domain-Containing 3 Protein
Depositing User:	Symplectic Admin
Date Deposited:	06 Jul 2023 13:47
Last Modified:	28 Oct 2023 22:50
DOI:	10.1371/journal.pone.0285323
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3171495