Hawkins, Paulina A, Chochua, Sopio, Lo, Stephanie W, Belman, Sophie, Antonio, Martin, Kwambana-Adams, Brenda, von Gottberg, Anne, du Plessis, Mignon, Cornick, Jen, Beall, Bernard et al (show 3 more authors)
(2023)
A global genomic perspective on the multidrug- resistant<i> Streptococcus</i><i> pneumoniae</i> 15A-CC63 sub- lineage following pneumococcal conjugate vaccine introduction.
MICROBIAL GENOMICS, 9 (4).
mgen000998-.
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A global genomic perspective on the multidrug-resistant iStreptococcus pneumoniaei 15A-CC63 sub-lineage following pneumococc.pdf - Open Access published version Download (1MB) | Preview |
Abstract
The introduction of pneumococcal conjugate vaccines (PCV7, PCV10, PCV13) around the world has proved successful in preventing invasive pneumococcal disease. However, immunization against <i>Streptococcus pneumoniae</i> has led to serotype replacement by non-vaccine serotypes, including serotype 15A. Clonal complex 63 (CC63) is associated with many serotypes and has been reported in association with 15A after introduction of PCVs. A total of 865 CC63 isolates were included in this study, from the USA (<i>n</i>=391) and a global collection (<i>n</i>=474) from 1998-2019 and 1995-2018, respectively. We analysed the genomic sequences to identify serotypes and penicillin-binding protein (PBP) genes 1A, 2B and 2X, and other resistance determinants, to predict minimum inhibitory concentrations (MICs) against penicillin, erythromycin, clindamycin, co-trimoxazole and tetracycline. We conducted phylogenetic and spatiotemporal analyses to understand the evolutionary history of the 15A-CC63 sub-lineage. Overall, most (89.5 %, <i>n</i>=247) pre-PCV isolates in the CC63 cluster belonged to serotype 14, with 15A representing 6.5 % of isolates. Conversely, serotype 14 isolates represented 28.2 % of post-PCV CC63 isolates (<i>n</i>=618), whilst serotype 15A isolates represented 65.4 %. Dating of the CC63 lineage determined the most recent common ancestor emerged in the 1980s, suggesting the 15A-CC63 sub-lineage emerged from its closest serotype 14 ancestor prior to the development of pneumococcal vaccines. This sub-lineage was predominant in the USA, Israel and China. Multidrug resistance (to three or more drug classes) was widespread among isolates in this sub-lineage. We show that the CC63 lineage is globally distributed and most of the isolates are penicillin non-susceptible, and thus should be monitored.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | antimicrobial resistance, pneumococcal conjugate vaccines, pneumococcus, serotype replacement |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 07 Jul 2023 14:57 |
| Last Modified: | 17 Mar 2024 16:32 |
| DOI: | 10.1099/mgen.0.000998 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3171535 |
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