Makepeace, Benjamin 
ORCID: 0000-0002-6100-6727
(2023)
Adjuvanted Fusion Protein Vaccine Induces Durable Immunity to Onchocerca volvulus in Mice and Non-Human Primates.
Vaccines, 11 (7).
1212-.
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Abstract
Onchocerciasis remains a debilitating neglected tropical disease. Due to the many challenges of current control methods, an effective vaccine against the causative agent Onchocerca volvulus is urgently needed. Mice and cynomolgus macaque non-human primates (NHPs) were immunized with a vaccine consisting of a fusion of two O. volvulus protein antigens, Ov-103 and Ov-RAL-2 (Ov-FUS-1), and three different adjuvants: Advax-CpG, alum, and AlT4. All vaccine formulations induced high antigen-specific IgG titers in both mice and NHPs. Challenging mice with O. volvulus L3 contained within subcutaneous diffusion chambers demonstrated that Ov-FUS-1/Advax-CpG-immunized animals developed protective immunity, durable for at least 11 weeks. Passive transfer of sera, collected at several time points, from both mice and NHPs immunized with Ov-FUS-1/Advax-CpG transferred protection to naïve mice. These results demonstrate that Ov-FUS-1 with the adjuvant Advax-CpG induces durable protective immunity against O. volvulus in mice and NHPs that is mediated by vaccine-induced humoral factors.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Onchocerca volvulus, vaccine, durability, adjuvant, mice, non-human primates, Advax, fusion protein, passive immunization, river blindness |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 10 Jul 2023 13:57 |
| Last Modified: | 11 Oct 2023 13:44 |
| DOI: | 10.3390/vaccines11071212 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3171573 |
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