Multispectral optoacoustic tomography is more sensitive than micro-computed tomography for tracking gold nanorod labelled mesenchymal stromal cells

Pichardo, Alejandra Hernandez, Littlewood, James, Taylor, Arthur ORCID: 0000-0003-2028-6694, Wilm, Bettina ORCID: 0000-0002-9245-993X, Levy, Raphael and Murray, Patricia ORCID: 0000-0003-1316-148X (2023) Multispectral optoacoustic tomography is more sensitive than micro-computed tomography for tracking gold nanorod labelled mesenchymal stromal cells. JOURNAL OF BIOPHOTONICS, 16 (10). e202300109-.

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Official URL: http://dx.doi.org/10.1002/jbio.202300109

Abstract

Tracking the fate of therapeutic cell types is important for assessing their safety and efficacy. Bioluminescence imaging (BLI) is an effective cell tracking technique, but poor spatial resolution means it has limited ability to precisely map cells in vivo in 3D. This can be overcome by using a bimodal imaging approach that combines BLI with a technique capable of generating high-resolution images. Here we compared the effectiveness of combining either multispectral optoacoustic tomography (MSOT) or micro-computed tomography (micro-CT) with BLI for tracking the fate of luciferase<sup>+</sup> human mesenchymal stromal cells (MSCs) labelled with gold nanorods. Following subcutaneous administration in mice, the MSCs could be readily detected with MSOT but not with micro-CT. We conclude that MSOT is more sensitive than micro-CT for tracking gold nanorod-labelled cells in vivo and depending on the route of administration, can be used effectively with BLI to track MSC fate in mice.

Item Type:	Article
Uncontrolled Keywords:	bioluminescence, cell tracking, gold nanorods, mesenchymal stromal cells, micro-CT, multispectral optoacoustic tomography
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences
Depositing User:	Symplectic Admin
Date Deposited:	13 Jul 2023 15:12
Last Modified:	30 Oct 2023 19:38
DOI:	10.1002/jbio.202300109
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3171690