Highly specific and non-invasive imaging of Piezo1-dependent activity across scales using GenEPi


Yaganoglu, Sine, Kalyviotis, Konstantinos, Vagena-Pantoula, Christina, Julich, Dorthe, Gaub, Benjamin M, Welling, Maaike, Lopes, Tatiana, Lachowski, Dariusz, Tang, See Swee, Hernandez, Armando Del Rio et al (show 8 more authors) (2023) Highly specific and non-invasive imaging of Piezo1-dependent activity across scales using GenEPi. NATURE COMMUNICATIONS, 14 (1). 4352-.

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Abstract

Mechanosensing is a ubiquitous process to translate external mechanical stimuli into biological responses. Piezo1 ion channels are directly gated by mechanical forces and play an essential role in cellular mechanotransduction. However, readouts of Piezo1 activity are mainly examined by invasive or indirect techniques, such as electrophysiological analyses and cytosolic calcium imaging. Here, we introduce GenEPi, a genetically-encoded fluorescent reporter for non-invasive optical monitoring of Piezo1-dependent activity. We demonstrate that GenEPi has high spatiotemporal resolution for Piezo1-dependent stimuli from the single-cell level to that of the entire organism. GenEPi reveals transient, local mechanical stimuli in the plasma membrane of single cells, resolves repetitive contraction-triggered stimulation of beating cardiomyocytes within microtissues, and allows for robust and reliable monitoring of Piezo1-dependent activity in vivo. GenEPi will enable non-invasive optical monitoring of Piezo1 activity in mechanochemical feedback loops during development, homeostatic regulation, and disease.

Item Type: Article
Uncontrolled Keywords: Cell Membrane, Ion Channels, Mechanotransduction, Cellular, Mechanical Phenomena
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User: Symplectic Admin
Date Deposited: 30 Aug 2023 08:38
Last Modified: 30 Aug 2023 08:38
DOI: 10.1038/s41467-023-40134-y
Open Access URL: https://www.nature.com/articles/s41467-023-40134-y
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3172396
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