Hinton, Jay 
ORCID: 0000-0003-2671-6026
(2023)
Diverse Durham collection phages demonstrate complex BREX defense responses.
Applied & Environmental Microbiology, 89 (9).
e0062323-.
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Abstract
Bacteriophages (phages) outnumber bacteria ten-to-one and cause infections at a rate of 1025 per second. The ability of phages to reduce bacterial populations makes them attractive alternative antibacterials for use in combating the rise in antimicrobial resistance. This effort may be hindered due to bacterial defenses such as Bacteriophage Exclusion (BREX) that have arisen from the constant evolutionary battle between bacteria and phages. For phages to be widely accepted as therapeutics in Western medicine, more must be understood about bacteria–phage interactions and the outcomes of bacterial phage defense. Here, we present the annotated genomes of 12 novel bacteriophage species isolated from water sources in Durham, UK, during undergraduate practical classes. The collection includes diverse species from across known phylogenetic groups. Comparative analyses of two novel phages from the collection suggest they may be founding members of a new genus. Using this Durham phage collection, we determined that particular BREX defense systems were likely to confer a varied degree of resistance against an invading phage. We concluded that the number of BREX target motifs encoded in the phage genome was not proportional to the degree of susceptibility.
| Item Type: | Article |
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| Uncontrolled Keywords: | bacteriophage, phylogenetics, autographiviridae, phage defense, BREX |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 07 Sep 2023 09:21 |
| Last Modified: | 05 Oct 2023 11:41 |
| DOI: | 10.1128/aem.00623-23 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3172586 |
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