Hicks, Melanie D, Ovaitt, Alyssa K, Morrison, Daniel R, Fleming, Jason C 
ORCID: 0000-0001-7963-1224, Jeyarajan, Hari, Greene, Benjamin, Sorace, Anna G, Patel, Juhi, Kasten, Benjamin B, Hartman, Yolanda E et al (show 3 more authors)
(2023)
Determination of Flap Survival Isolated From Wound Bed Vasculature Using a Murine Axial Flap Model.
Ear, Nose & Throat Journal.
1455613231183392-.
Abstract
<jats:p> Background: Axial pattern flaps are a common reconstructive option following resection of soft tissue malignancies. We determine the early dependence of an axial flap on wound bed vasculature by isolating the underlying wound bed and depriving contact with the overlying flap. Materials and Methods: Mice were divided into 5 groups: No silicone (n = 7), silicone in the proximal 50% of the wound bed (n = 8), silicone in the distal 50% of the wound bed (n = 5), silicone over the full length of the wound bed with pedicle preservation (n = 5), and silicone over the full length of the wound bed with pedicle sacrifice (n = 5). The pedicle was the lateral thoracic artery. Daily photographs were taken, and the percent of viable flap was determined using ImageJ© software (public domain JAVA image processing program, National Institute of Health, Bethesda, MA). Percent flap viability for each group was compared to the no silicone group, which acted as the reference. Results: Mean differences in percent flap necrotic area (with 95% confidence interval) compared to the no silicone group were −0.15% (−15.09 to 14.09), 2.07% (−5.26 to 9.39), 2.98% (−10.98 to 16.94), and 14.21% (0.48 to 27.94) for the full-length silicone with preserved pedicle, proximal silicone, distal silicone, and full-length silicone with sacrificed pedicle groups, respectively. The full-length silicone with sacrificed pedicle group had a significant difference in flap viability ( P = .045) compared to the no silicone group. Conclusion: We investigate the role of the wound bed vasculature in a murine axial flap model and demonstrate that the wound bed vasculature is not essential for early distal flap survival. </jats:p>
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | animal model, axial flap, necrosis, surgery |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 27 Sep 2023 10:50 |
| Last Modified: | 15 Mar 2024 19:44 |
| DOI: | 10.1177/01455613231183392 |
| Open Access URL: | https://doi.org/10.1177/01455613231183392 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3173114 |
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