Identification of d-arabinan-degrading enzymes in mycobacteria

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Al-Jourani, Omar, Benedict, Samuel T, Ross, Jennifer, Layton, Abigail J, van der Peet, Phillip, Marando, Victoria M, Bailey, Nicholas P, Heunis, Tiaan, Manion, Joseph, Mensitieri, Francesca et al (show 16 more authors) , Franklin, Aaron, Abellon-Ruiz, Javier, Oram, Sophia L, Parsons, Lauren, Cartmell, Alan ORCID: 0000-0002-5512-249X, Wright, Gareth SA, Basle, Arnaud, Trost, Matthias, Henrissat, Bernard, Munoz-Munoz, Jose, Hirt, Robert P, Kiessling, Laura L, Lovering, Andrew L, Williams, Spencer J, Lowe, Elisabeth C and Moynihan, Patrick J (2023) Identification of d-arabinan-degrading enzymes in mycobacteria. NATURE COMMUNICATIONS, 14 (1). 2233-.

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Abstract

Bacterial cell growth and division require the coordinated action of enzymes that synthesize and degrade cell wall polymers. Here, we identify enzymes that cleave the D-arabinan core of arabinogalactan, an unusual component of the cell wall of Mycobacterium tuberculosis and other mycobacteria. We screened 14 human gut-derived Bacteroidetes for arabinogalactan-degrading activities and identified four families of glycoside hydrolases with activity against the D-arabinan or D-galactan components of arabinogalactan. Using one of these isolates with exo-D-galactofuranosidase activity, we generated enriched D-arabinan and used it to identify a strain of Dysgonomonas gadei as a D-arabinan degrader. This enabled the discovery of endo- and exo-acting enzymes that cleave D-arabinan, including members of the DUF2961 family (GH172) and a family of glycoside hydrolases (DUF4185/GH183) that display endo-D-arabinofuranase activity and are conserved in mycobacteria and other microbes. Mycobacterial genomes encode two conserved endo-D-arabinanases with different preferences for the D-arabinan-containing cell wall components arabinogalactan and lipoarabinomannan, suggesting they are important for cell wall modification and/or degradation. The discovery of these enzymes will support future studies into the structure and function of the mycobacterial cell wall.

Item Type:	Article
Uncontrolled Keywords:	Cell Wall, Humans, Mycobacterium tuberculosis, Glycoside Hydrolases, Polysaccharides
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences
Depositing User:	Symplectic Admin
Date Deposited:	05 Oct 2023 10:02
Last Modified:	05 Oct 2023 11:42
DOI:	10.1038/s41467-023-37839-5
Open Access URL:	https://doi.org/10.1038/s41467-023-37839-5
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3173442