Prozzillo, Yuri, Fattorini, Gaia, Santopietro, Maria Virginia, Suglia, Luigi, Ruggiero, Alessandra 
ORCID: 0000-0001-6436-8814, Ferreri, Diego and Messina, Giovanni
(2020)
Targeted Protein Degradation Tools: Overview and Future Perspectives.
BIOLOGY-BASEL, 9 (12).
E421-.
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Targeted Protein Degradation Tools Overview and Future Perspectives. .pdf - Open Access published version Download (4MB) | Preview |
Abstract
Targeted protein inactivation (TPI) is an elegant approach to investigate protein function and its role in the cellular landscape, overcoming limitations of genetic perturbation strategies. These systems act in a reversible manner and reduce off-target effects exceeding the limitations of CRISPR/Cas9 and RNA interference, respectively. Several TPI have been developed and wisely improved, including compartment delocalization tools and protein degradation systems. However, unlike chemical tools such as PROTACs (PROteolysis TArgeting Chimeras), which work in a wild-type genomic background, TPI technologies require adding an aminoacidic signal sequence (tag) to the protein of interest (POI). On the other hand, the design and optimization of PROTACs are very laborious and time-consuming. In this review, we focus on anchor-away, deGradFP, auxin-inducible degron (AID) and dTAG technologies and discuss their recent applications and advances. Finally, we propose nano-grad, a novel nanobody-based protein degradation tool, which specifically proteolyzes endogenous tag-free target protein.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | anchor-away, deGradFP, degron, dTAG, FKBP12, Lindau (VHL), nanobody, nano-grad, targeted protein degradation (TPD), targeted protein inactivation (TPI), von Hippel– |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 09 Oct 2023 09:52 |
| Last Modified: | 09 Oct 2023 09:53 |
| DOI: | 10.3390/biology9120421 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3173529 |
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