Anderson, James R 
ORCID: 0000-0003-0489-7997, Johnson, Emily, Jenkins, Rosalind ORCID: 0000-0002-3730-1136, Jacobsen, Stine ORCID: 0000-0002-2380-4576, Green, Daniel, Walters, Marie, Bundgaard, Louise, Hausmans, Bas AC, van den Akker, Guus ORCID: 0000-0002-7071-1487, Welting, Tim JM et al (show 5 more authors)
(2023)
Multi-Omic Temporal Landscape of Plasma and Synovial Fluid-Derived Extracellular Vesicles Using an Experimental Model of Equine Osteoarthritis.
International journal of molecular sciences, 24 (19).
14888-.
Abstract
Extracellular vesicles (EVs) contribute to osteoarthritis pathogenesis through their release into joint tissues and synovial fluid. Synovial fluid-derived EVs have the potential to be direct biomarkers in the causal pathway of disease but also enable understanding of their role in disease progression. Utilizing a temporal model of osteoarthritis, we defined the changes in matched synovial fluid and plasma-derived EV small non-coding RNA and protein cargo using sequencing and mass spectrometry. Data exploration included time series clustering, factor analysis and gene enrichment interrogation. Chondrocyte signalling was analysed using luciferase-based transcription factor activity assays. EV protein cargo appears to be more important during osteoarthritis progression than small non-coding RNAs. Cluster analysis revealed plasma-EVs represented a time-dependent response to osteoarthritis induction associated with supramolecular complexes. Clusters for synovial fluid-derived EVs were associated with initial osteoarthritis response and represented immune/inflammatory pathways. Factor analysis for plasma-derived EVs correlated with day post-induction and were primarily composed of proteins modulating lipid metabolism. Synovial fluid-derived EVs factors represented intermediate filament and supramolecular complexes reflecting tissue repair. There was a significant interaction between time and osteoarthritis for CRE, NFkB, SRE, SRF with a trend for osteoarthritis synovial fluid-derived EVs at later time points to have a more pronounced effect.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Synovial Fluid, Animals, Horses, Osteoarthritis, Models, Theoretical, Extracellular Vesicles, Multiomics |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 17 Oct 2023 15:20 |
| Last Modified: | 02 Nov 2023 23:14 |
| DOI: | 10.3390/ijms241914888 |
| Open Access URL: | https://doi.org/10.3390/ijms241914888 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3173835 |
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