<i>Helicobacter pylori</i> extract induces purified neutrophils to produce reactive oxygen species only in the presence of plasma.


Thai, Tran Duong, Chuenchom, Chalida, Donsa, Wachiraporn, Faksri, Kiatichai, Sripa, Banchob, Edwards, Steven W ORCID: 0000-0002-7074-0552 and Salao, Kanin ORCID: 0000-0003-4731-9556 (2023) <i>Helicobacter pylori</i> extract induces purified neutrophils to produce reactive oxygen species only in the presence of plasma. Biomedical reports, 19 (6). 89-.

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Abstract

<i>H. pylori</i> is a bacterial pathogen infecting over half of the world's population and induces several gastric and extra-gastric diseases through its various virulence factors, especially <i>cag</i>A. These factors may be released from the bacteria during interactions with host immune cells. Neutrophils play key roles in innate immunity, and their activity is regulated by plasma factors, which can alter how these cells may interact with pathogens. The aim of the present study was to determine whether purified neutrophils could produce reactive oxygen species (ROS), one of the key functions of their anti-microbial functions, in response to extracts of <i>cagA</i>+ and <i>cagA</i>- <i>H. pylori</i>. Extracts from either <i>cagA+</i> or <i>cagA- H. pylori</i> were co-cultured with human neutrophils in the presence or absence of plasma, and the neutrophil ROS production was measured. In the absence of plasma, extracts from <i>cag</i>A+ and <i>cag</i>A- <i>H. pylori</i> did not induce neutrophil ROS production, whereas in the presence of plasma, extracts from both <i>cagA</i>+ and <i>cagA</i>- <i>H. pylori</i>-induced ROS production. Furthermore, when peripheral blood mononuclear cells (PBMCs) were added to the purified neutrophils in the absence of plasma, there was no neutrophil ROS production after challenging with extracts from either <i>cagA</i>+ or <i>cagA</i>- <i>H. pylori</i>. Thus, it is suggested that plasma contains immunological components that change the responsiveness of neutrophils, such that when neutrophils encounter the bacterial antigens in <i>H. pylori</i> extracts, they become activated and produce ROS. This study also revealed a potential novel immunopathogenic pathway by which <i>cag</i>A activation of neutrophils contributed to inflammatory damage.

Item Type: Article
Uncontrolled Keywords: Helicobacter pylori, cagA, plasma, purified neutrophils, reactive oxygen species
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences
Depositing User: Symplectic Admin
Date Deposited: 20 Nov 2023 15:07
Last Modified: 08 Dec 2023 15:27
DOI: 10.3892/br.2023.1671
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3176899
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