A two-step DNA barcoding approach for delimiting moth species: moths of Dongling Mountain (Beijing, China) as a case study


Jin, Qian, Hu, Xi-Min, Han, Hui-Lin, Chen, Fen, Cai, Wei-Jia, Ruan, Qian-Qian, Liu, Bo, Luo, Gui-Jie, Wang, Hao, Liu, Xu et al (show 4 more authors) (2018) A two-step DNA barcoding approach for delimiting moth species: moths of Dongling Mountain (Beijing, China) as a case study. SCIENTIFIC REPORTS, 8 (1). 14256-.

Access the full-text of this item by clicking on the Open Access link.
[img] PDF
A two-step DNA barcoding approach for delimiting moth species moths of Dongling Mountain (Beijing, China) as a case study.pdf - Open Access published version
Download (1MB) | Preview

Abstract

DNA barcoding, based on a fragment of cytochrome c oxidase I (COI) mtDNA, is as an effective molecular tool for identification, discovery, and biodiversity assessment for most animals. However, multiple gene markers coupled with more sophisticated analytical approaches may be necessary to clarify species boundaries in cases of cryptic diversity or morphological plasticity. Using 339 moths collected from mountains surrounding Beijing, China, we tested a pipeline consisting of two steps: (1) rapid morphospecies sorting and screening of the investigated fauna with standard COI barcoding approaches; (2) additional analyses with multiple molecular markers for those specimens whose morphospecies and COI barcode grouping were incongruent. In step 1, 124 morphospecies were delimited into 116 barcode units, with 90% of the conflicts being associated with specimens identified to the genus Hypena. In step 2, 55 individuals representing all 12 Hypena morphospecies were analysed using COI, COII, 28S, EF-1a, Wgl sequences or their combinations with the BPP (Bayesian Phylogenetics and Phylogeography) multigene species delimitation method. The multigene analyses supported the delimitation of 5 species, consistent with the COI analysis. We conclude that a two-step barcoding analysis pipeline is able to rapidly characterize insect biodiversity and help to elucidate species boundaries for taxonomic complexes without jeopardizing overall project efficiency by substantially increasing analytical costs.

Item Type: Article
Uncontrolled Keywords: Animals, Moths, DNA, Mitochondrial, Bayes Theorem, Biodiversity, Species Specificity, China, DNA Barcoding, Taxonomic, Phylogeography
Depositing User: Symplectic Admin
Date Deposited: 30 Nov 2023 16:34
Last Modified: 30 Nov 2023 16:34
DOI: 10.1038/s41598-018-32123-9
Open Access URL: https://doi.org/10.1038/s41598-018-32123-9
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3177095
Dimensions
Altmetric
CORE (COnnecting REpositories)