Thompson, Beth, Dilly-Penchala, Sujan, Amara, Alieu 
ORCID: 0000-0002-1137-2948, Reynolds, Helen ORCID: 0000-0001-7443-4520, Khoo, Saye ORCID: 0000-0002-2769-0967 and Else, Laura
(2023)
Application of novel plasma separation filter cards for quantification of nucleoside/nucleotide reverse transcriptase inhibitor di/triphosphates in dried blood spot using LC-MS.
BIOANALYSIS, 15 (13).
pp. 739-756.
Abstract
<b>Background:</b> A rapid and sensitive LC-MS method has been developed and validated for the quantification of nucleoside di/triphosphates using a novel plasma separation card (HemaSep). <b>Materials & methods:</b> Cards were spotted with whole blood and stored at -80°C. Metabolites were extracted using 70:30 MeOH:20% formic acid, followed by weak anion exchange SPE and eluted using a Biobasic-AX column. Quantification was performed using a triple quadrupole mass spectrometer with a calibration range of 1.25-250 pmol/sample. <b>Results:</b> The recovery of metabolites was high (>93%). Precision and accuracy were acceptable and metabolites remained stable on the card after 29 days (stored at ambient temperature). <b>Conclusion:</b> HemaSep dried blood spots are a useful microsampling tool and offer an alternative to liquid plasma as they maintain stability over time.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | adherence, antiretrovirals, dried blood spots, emtricitabine triphosphate, lamivudine triphosphate, LC-MS, tenofovir diphosphate |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 14 Dec 2023 08:59 |
| Last Modified: | 14 Dec 2023 09:00 |
| DOI: | 10.4155/bio-2023-0057 |
| Open Access URL: | https://doi.org/10.4155/bio-2023-0057 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3177368 |
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