Aruta, Maria Grazia, Lari, Elisa, De Simone, Daniele, Semplici, Bianca, Semplici, Claudia, Dale, Helen, Chirwa, Esmelda, Kadwala, Innocent, Mbewe, Maurice, Banda, Happy et al (show 9 more authors)
(2023)
Characterization of Enzyme-Linked Immunosorbent Assay (ELISA) for Quantification of Antibodies against <i>Salmonella</i> Typhimurium and <i>Salmonella</i> Enteritidis O-Antigens in Human Sera.
BIOTECH, 12 (3).
54-.
Abstract
Nontyphoidal Salmonella (NTS) is a leading cause of morbidity and mortality caused by enteric pathogens worldwide in both children and adults, and vaccines are not yet available. The measurement of antigen-specific antibodies in the sera of vaccinated or convalescent individuals is crucial to understand the incidence of disease and the immunogenicity of vaccine candidates. A solid and standardized assay used to determine the level of specific anti-antigens IgG is therefore of paramount importance. In this work, we presented the characterization of a customized enzyme-linked immunosorbent assay (ELISA) with continuous readouts and a standardized definition of EU/mL. We assessed various performance parameters: standard curve accuracy, dilutional linearity, intermediate precision, specificity, limits of blanks, and quantification. The simplicity of the assay, its high sensitivity and specificity coupled with its low cost and the use of basic consumables and instruments without the need of high automation makes it suitable for transfer and application to different laboratories, including resource-limiting settings where the disease is endemic. This ELISA is, therefore, fit for purpose to be used for quantification of antibodies against <i>Salmonella</i> Typhimurium and <i>Salmonella</i> Enteritidis O-antigens in human samples, both for vaccine clinical trials and large sero-epidemiological studies.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | antibodies, Enzyme-Linked Immunosorbent Assay (ELISA), Generalized Modules for Membrane Antigens (GMMA), human sera, iNTS, non-typhoidal Salmonella, vaccine |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 14 Dec 2023 09:23 |
| Last Modified: | 14 Dec 2023 09:32 |
| DOI: | 10.3390/biotech12030054 |
| Open Access URL: | https://doi.org/10.3390/biotech12030054 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3177385 |
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