Mathematical modelling and deep learning algorithms to automate assessment of single and digitally multiplexed immunohistochemical stains in tumoural stroma.

Burrows, Liam ORCID: 0000-0002-6910-6693, Sculthorpe, Declan, Zhang, Hongrun, Rehman, Obaid, Mukherjee, Abhik and Chen, Ke ORCID: 0000-0002-6093-6623 (2024) Mathematical modelling and deep learning algorithms to automate assessment of single and digitally multiplexed immunohistochemical stains in tumoural stroma. Journal of pathology informatics, 15. 100351-.

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Official URL: http://dx.doi.org/10.1016/j.jpi.2023.100351

Abstract

Whilst automated analysis of immunostains in pathology research has focused predominantly on the epithelial compartment, automated analysis of stains in the stromal compartment is challenging and therefore requires time-consuming pathological input and guidance to adjust to tissue morphometry as perceived by pathologists. This study aimed to develop a robust method to automate stromal stain analyses using 2 of the commonest stromal stains (SMA and desmin) employed in clinical pathology practice as examples. An effective computational method capable of automatically assessing and quantifying tumour-associated stromal stains was developed and applied on cores of colorectal cancer tissue microarrays. The methodology combines both mathematical models and deep learning techniques with the former requiring no training data and the latter as many inputs as possible. The novel mathematical model was used to produce a digital double marker overlay allowing for fast automated digital multiplex analysis of stromal stains. The results show that deep learning methodologies in combination with mathematical modelling allow for an accurate means of quantifying stromal stains whilst also opening up new possibilities of digital multiplex analyses.

Item Type:	Article
Uncontrolled Keywords:	Digital multiplex, Digital pathology, Machine learning, Mathematical modelling, Stromal stain, Tissue microarrays
Divisions:	Faculty of Science and Engineering > School of Physical Sciences
Depositing User:	Symplectic Admin
Date Deposited:	18 Dec 2023 16:54
Last Modified:	13 Jan 2024 01:57
DOI:	10.1016/j.jpi.2023.100351
Open Access URL:	https://doi.org/10.1016/j.jpi.2023.100351
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3177515