Identification of two distinct phylogenomic lineages and model strains for the understudied cystic fibrosis lung pathogen <i>Burkholderia multivorans</i>.


Parfitt, Kasia M, Green, Angharad E ORCID: 0000-0002-8683-8191, Connor, Thomas R, Neill, Daniel R ORCID: 0000-0002-7911-8153 and Mahenthiralingam, Eshwar (2023) Identification of two distinct phylogenomic lineages and model strains for the understudied cystic fibrosis lung pathogen <i>Burkholderia multivorans</i>. Microbiology (Reading, England), 169 (8). 001366-.

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Abstract

<i>Burkholderia multivorans</i> is the dominant <i>Burkholderia</i> pathogen recovered from lung infection in people with cystic fibrosis. However, as an understudied pathogen there are knowledge gaps in relation to its population biology, phenotypic traits and useful model strains. A phylogenomic study of <i>B. multivorans</i> was undertaken using a total of 283 genomes, of which 73 were sequenced and 49 phenotypically characterized as part of this study. Average nucleotide identity analysis (ANI) and phylogenetic alignment of core genes demonstrated that the <i>B. multivorans</i> population separated into two distinct evolutionary clades, defined as lineage 1 (<i>n</i>=58 genomes) and lineage 2 (<i>n</i>=221 genomes). To examine the population biology of <i>B. multivorans</i>, a representative subgroup of 77 <i>B. multivorans</i> genomes (28 from the reference databases and the 49 novel short-read genome sequences) were selected based on multilocus sequence typing (MLST), isolation source and phylogenetic placement criteria. Comparative genomics was used to identify <i>B. multivorans</i> lineage-specific genes - <i>ghrB_1</i> in lineage 1 and <i>glnM_2</i> in lineage 2 - and diagnostic PCRs targeting them were successfully developed. Phenotypic analysis of 49 representative <i>B. multivorans</i> strains showed considerable inter-strain variance, but the majority of the isolates tested were motile and capable of biofilm formation. A striking absence of <i>B. multivorans</i> protease activity <i>in vitro</i> was observed, but no lineage-specific phenotypic differences were demonstrated. Using phylogenomic and phenotypic criteria, three model <i>B. multivorans</i> CF strains were identified, BCC0084 (lineage 1), BCC1272 (lineage 2a) and BCC0033 lineage 2b, and their complete genome sequences determined. <i>B. multivorans</i> CF strains BCC0033 and BCC0084, and the environmental reference strain, ATCC 17616, were all capable of short-term survival within a murine lung infection model. By mapping the population biology, identifying lineage-specific PCRs and model strains, we provide much needed baseline resources for future studies of <i>B. multivorans</i>.

Item Type: Article
Uncontrolled Keywords: Animals, Mice, Inbred BALB C, Mice, Burkholderia, Burkholderia Infections, Cystic Fibrosis, Phylogeny, Genome, Bacterial, Female, Multilocus Sequence Typing
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences
Depositing User: Symplectic Admin
Date Deposited: 31 Jan 2024 11:50
Last Modified: 15 Mar 2024 00:42
DOI: 10.1099/mic.0.001366
Open Access URL: https://doi.org/10.1099/mic.0.001366
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3178193
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