Moreno-Cabezuelo, Jose Angel, Gomez-Baena, Guadalupe 
ORCID: 0000-0003-3796-3874, Diez, Jesus and Garcia-Fernandez, Jose Manuel
(2023)
Integrated Proteomic and Metabolomic Analyses Show Differential Effects of Glucose Availability in Marine <i>Synechococcus</i> and <i>Prochlorococcus</i>.
MICROBIOLOGY SPECTRUM, 11 (2).
e0327522-.
Abstract
We compared changes induced by the addition of 100 nM and 5 mM glucose on the proteome and metabolome complements in <i>Synechococcus</i> sp. strains WH8102, WH7803, and BL107 and <i>Prochlorococcus</i> sp. strains MED4, SS120, and MIT9313, grown either under standard light conditions or in darkness. Our results suggested that glucose is metabolized by these cyanobacteria, using primarily the oxidative pentoses and Calvin pathways, while no proof was found for the involvement of the Entner-Doudoroff pathway in this process. We observed differences in the effects of glucose availability, both between genera and between <i>Prochlorococcus</i> MED4 and SS120 strains, which might be related to their specific adaptations to the environment. We found evidence for fermentation in <i>Prochlorococcus</i> sp. strain SS120 and <i>Synechococcus</i> sp. strain WH8102 after 5 mM glucose addition. Our results additionally suggested that marine cyanobacteria can detect nanomolar glucose concentrations in the environment and that glucose might be used to sustain metabolism under darkness. Furthermore, the KaiB and KaiC proteins were also affected in <i>Synechococcus</i> sp. WH8102, pointing to a direct link between glucose assimilation and circadian rhythms in marine cyanobacteria. In conclusion, our study provides a wide overview on the metabolic effects induced by glucose availability in representative strains of the diverse marine picocyanobacteria, providing further evidence for the importance of mixotrophy in marine picocyanobacteria. <b>IMPORTANCE</b> Glucose uptake by marine picocyanobacteria has been previously described and strongly suggests they are mixotrophic organisms (capable of using energy from the sun to make organic matter, but also to directly use organic matter from the environment when available). However, a detailed analysis of the effects of glucose addition on the proteome and metabolome of these microorganisms had not been carried out. Here, we analyzed three <i>Prochlorococcus</i> sp. and three <i>Synechococcus</i> sp. strains which were representative of several marine picocyanobacterial clades. We observed differential features in the effects of glucose availability, depending on both the genus and strain; our study illuminated the strategies utilized by these organisms to metabolize glucose and showed unexpected links to other pathways, such as circadian regulation. Furthermore, we found glucose addition had profound effects in the microbiome, favoring the growth of coexisting heterotrophic bacteria.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | glucose, marine Synechococcus, metabolomics, mixotrophy, Prochlorococcus, proteomics |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 31 Jan 2024 11:49 |
| Last Modified: | 31 Jan 2024 11:49 |
| DOI: | 10.1128/spectrum.03275-22 |
| Open Access URL: | https://doi.org/10.1128/spectrum.03275-22 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3178208 |
Dimensions
Dimensions
