Menzies, Stefanie K 
ORCID: 0000-0002-9273-9296, Dawson, Charlotte A, Crittenden, Edouard, Edge, Rebecca J, Hall, Steven R, Alsolaiss, Jaffer, Wilkinson, Mark C, Casewell, Nicholas R, Harrison, Robert A and Ainsworth, Stuart
(2022)
Virus-like particles displaying conserved toxin epitopes stimulate polyspecific, murine antibody responses capable of snake venom recognition.
SCIENTIFIC REPORTS, 12 (1).
11328-.
Abstract
Antivenom is currently the first-choice treatment for snakebite envenoming. However, only a low proportion of antivenom immunoglobulins are specific to venom toxins, resulting in poor dose efficacy and potency. We sought to investigate whether linear venom epitopes displayed on virus like particles can stimulate an antibody response capable of recognising venom toxins from diverse medically important species. Bioinformatically-designed epitopes, corresponding to predicted conserved regions of group I phospholipase A<sub>2</sub> and three finger toxins, were engineered for display on the surface of hepatitis B core antigen virus like particles and used to immunise female CD1 mice over a 14 weeks. Antibody responses to all venom epitope virus like particles were detectable by ELISA by the end of the immunisation period, although total antibody and epitope specific antibody titres were variable against the different epitope immunogens. Immunoblots using pooled sera demonstrated recognition of various venom components in a diverse panel of six elapid venoms, representing three continents and four genera. Insufficient antibody yields precluded a thorough assessment of the neutralising ability of the generated antibodies, however we were able to test polyclonal anti-PLA<sub>2</sub> IgG from three animals against the PLA<sub>2</sub> activity of Naja nigricollis venom, all of which showed no neutralising ability. This study demonstrates proof-of-principle that virus like particles engineered to display conserved toxin linear epitopes can elicit specific antibody responses in mice which are able to recognise a geographically broad range of elapid venoms.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Animals, Mice, Snake Venoms, Elapid Venoms, Antivenins, Epitopes, Antibody Formation, Female, Toxins, Biological |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology Faculty of Health and Life Sciences > Tech, Infrastructure and Environmental Directorate |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 02 Feb 2024 11:06 |
| Last Modified: | 02 Feb 2024 11:07 |
| DOI: | 10.1038/s41598-022-13376-x |
| Open Access URL: | https://doi.org/10.1038/s41598-022-13376-x |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3178353 |
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