People with obesity exhibit losses in muscle proteostasis that are partly improved by exercise training.

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Srisawat, Kanchana, Stead, Connor A, Hesketh, Katie, Pogson, Mark ORCID: 0000-0002-2116-7200, Strauss, Juliette A, Cocks, Matt, Siekmann, Ivo, Phillips, Stuart M, Lisboa, Paulo J, Shepherd, Sam et al (show 1 more authors) and Burniston, Jatin G (2023) People with obesity exhibit losses in muscle proteostasis that are partly improved by exercise training. Proteomics. e2300395-e2300395.

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Official URL: http://dx.doi.org/10.1002/pmic.202300395

Abstract

This pilot experiment examines if a loss in muscle proteostasis occurs in people with obesity and whether endurance exercise positively influences either the abundance profile or turnover rate of proteins in this population. Men with (n = 3) or without (n = 4) obesity were recruited and underwent a 14-d measurement protocol of daily deuterium oxide (D<sub>2</sub> O) consumption and serial biopsies of vastus lateralis muscle. Men with obesity then completed 10-weeks of high-intensity interval training (HIIT), encompassing 3 sessions per week of cycle ergometer exercise with 1 min intervals at 100% maximum aerobic power interspersed by 1 min recovery periods. The number of intervals per session progressed from 4 to 8, and during weeks 8-10 the 14-d measurement protocol was repeated. Proteomic analysis detected 352 differences (p < 0.05, false discovery rate < 5%) in protein abundance and 19 (p < 0.05) differences in protein turnover, including components of the ubiquitin-proteasome system. HIIT altered the abundance of 53 proteins and increased the turnover rate of 22 proteins (p < 0.05) and tended to benefit proteostasis by increasing muscle protein turnover rates. Obesity and insulin resistance are associated with compromised muscle proteostasis, which may be partially restored by endurance exercise.

Item Type:	Article
Uncontrolled Keywords:	biosynthetic labelling, deuterium oxide, fractional synthesis rate, heat shock proteins, heavy water, muscle protein synthesis, protein turnover, proteomics, skeletal muscle, ubiquitin proteasome system
Divisions:	Faculty of Humanities and Social Sciences > School of the Arts
Depositing User:	Symplectic Admin
Date Deposited:	06 Feb 2024 08:23
Last Modified:	06 Feb 2024 08:35
DOI:	10.1002/pmic.202300395
Open Access URL:	https://analyticalsciencejournals.onlinelibrary.wi...
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3178401