Sortilin Expression Levels and Peripheral Immunity: A Potential Biomarker for Segregation between Parkinson's Disease Patients and Healthy Controls.

Georgoula, Maria, Ntavaroukas, Panagiotis, Androutsopoulou, Anastasia, Xiromerisiou, Georgia ORCID: 0000-0001-7162-3588, Kalala, Fani, Speletas, Matthaios ORCID: 0000-0003-1287-7734, Asprodini, Eftihia, Vasilaki, Anna ORCID: 0000-0002-0852-4883 and Papoutsopoulou, Stamatia ORCID: 0000-0001-6665-8508 (2024) Sortilin Expression Levels and Peripheral Immunity: A Potential Biomarker for Segregation between Parkinson's Disease Patients and Healthy Controls. International journal of molecular sciences, 25 (3). 1791-.

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Abstract

Parkinson's disease (PD) is characterized by substantial phenotypic heterogeneity that limits the disease prognosis and patient's counseling, and complicates the design of further clinical trials. There is an unmet need for the development and validation of biomarkers for the prediction of the disease course. In this study, we utilized flow cytometry and in vitro approaches on peripheral blood cells and isolated peripheral blood mononuclear cell (PBMC)-derived macrophages to characterize specific innate immune populations in PD patients versus healthy donors. We found a significantly lower percentage of B lymphocytes and monocyte populations in PD patients. Monocytes in PD patients were characterized by a higher CD40 expression and on-surface expression of the type I membrane glycoprotein sortilin, which showed a trend of negative correlation with the age of the patients. These results were further investigated in vitro on PBMC-derived macrophages, which, in PD patients, showed higher sortilin expression levels compared to cells from healthy donors. The treatment of PD-derived macrophages with oxLDL led to higher foam cell formation compared to healthy donors. In conclusion, our results support the hypothesis that surface sortilin expression levels on human peripheral monocytes may potentially be utilized as a marker of Parkinson's disease and may segregate the sporadic versus the genetically induced forms of the disease.

Item Type:	Article
Uncontrolled Keywords:	Leukocytes, Mononuclear, Humans, Parkinson Disease, Adaptor Proteins, Vesicular Transport, Biomarkers
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences
Depositing User:	Symplectic Admin
Date Deposited:	20 Feb 2024 11:27
Last Modified:	26 Feb 2024 03:21
DOI:	10.3390/ijms25031791
Open Access URL:	https://doi.org/10.3390/ijms25031791
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3178819