Hajidariyor, Tasnim, Nuntawad, Nutchanon, Somsaen, Panadda, Prukdamrongchai, Raninnart, Cherdchoo, Harit, Posoknistakul, Pattaraporn, Khemthong, Pongtanawat, Wanmolee, Wanwitoo, Arjfuk, Pariyapat, Pongchaikul, Pisut 
ORCID: 0000-0001-8757-3554 et al (show 3 more authors)
(2023)
Cryo-Induced Cellulose-Based Nanogel from <i>Elaeis guineensis</i> for Antibiotic Delivery Platform.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 24 (2).
1230-.
Abstract
Cryo-induced hydrogel from cellulose is a new class of biomaterials for drug delivery, cell delivery, bone and skin tissue engineering for cell proliferation and regeneration applications. This research aimed to synthesize cryo-induced hydrogel from cellulose and carboxymethyl cellulose (CMC) produced from empty bunch's cell wall of <i>Elaeis guineensis</i>. First, the experiment was to produce cellulose-rich material using hot-compressed water extraction followed by alkaline delignification and bleaching with H<sub>2</sub>O<sub>2</sub>. The obtained bleached EFB cellulose was used as the substrate for CMC, and the optimal condition with the highest degree of carboxyl substitution (DS) of 0.75 was achieved when varying NaOH and monochloroacetic acid concentration as well as etherification temperature using fractional factorial design. For cryogelation study, hydrogels were synthesized from cellulose, CMC and beta-cyclodextrin (β-CD) by dissolving cellulose-based matrix in a NaOH/urea system, and the cellulose (CEL) solution was frozen spontaneously at -40 °C followed by high speed mixing to loosen cellulose fibrils. Epichlorohydrin (ECH) and Polyethylene glycol diglycidyl ether (PEGDE) were used as a cross-linker. First, the ratio of cellulose and CMC with different amounts of ECH was investigated, and subsequently the proper ratio was further studied by adding different crosslinkers and matrices, i.e., CMC and β-CD. From the result, the ECH crosslinked CMC-CEL (E-CMC-CEL) gel had the highest swelling properties of 5105% with the average pore size of lyophilized hydrogel of 300 µm. In addition, E-CMC-CEL gel had the highest loading and release capability of tetracycline in buffer solution at pH 7.4 and 3.2. At pH 7.4, tetracycline loading and release properties of E-CMC-CEL gel were 65.85 mg g<sup>-1</sup> dry hydrogel and 46.48 mg g<sup>-1</sup> dry hydrogel (70.6% cumulative release), respectively. However, at pH 3.2, the loading and release capabilities of Tetracycline were moderately lower at 16.25 mg g<sup>-1</sup> dry hydrogel and 5.06 mg g<sup>-1</sup> dry hydrogel, respectively. The findings presented that E-CMC-CEL hydrogel was a suitable material for antibiotic tetracycline drug carrying platform providing successful inhibitory effect on <i>Staphylococcus aureus</i>, <i>Escherichia coli</i> and <i>Pseudomonas aeruginosa</i>, respectively.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | antimicrobial activity, beta-cyclodextrin, carboxymethyl cellulose (CMC) synthesis, cellulose production, cryogelation, delignification, drug delivery, hydrogel, oil palm empty fruit bunch, pH controlled release, tetracycline |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 28 Feb 2024 10:11 |
| Last Modified: | 28 Feb 2024 10:11 |
| DOI: | 10.3390/ijms24021230 |
| Open Access URL: | https://doi.org/10.3390/ijms24021230 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3178929 |
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