Force-Dependent Binding Constants.

Wang, Yinan, Yan, Jie ORCID: 0000-0002-8555-7291 and Goult, Benjamin T ORCID: 0000-0002-3438-2807 (2019) Force-Dependent Binding Constants. Biochemistry, 58 (47). pp. 4696-4709.

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Official URL: http://dx.doi.org/10.1021/acs.biochem.9b00453

Abstract

Life is an emergent property of transient interactions between biomolecules and other organic and inorganic molecules that somehow leads to harmony and order. Measurement and quantitation of these biological interactions are of value to scientists and are major goals of biochemistry, as affinities provide insight into biological processes. In an organism, these interactions occur in the context of forces and the need for a consideration of binding affinities in the context of a changing mechanical landscape necessitates a new way to consider the biochemistry of protein-protein interactions. In the past few decades, the field of mechanobiology has exploded, as both the appreciation of, and the technical advances required to facilitate the study of, how forces impact biological processes have become evident. The aim of this review is to introduce the concept of force dependence of biomolecular interactions and the requirement to be able to measure force-dependent binding constants. The focus of this discussion will be on the mechanotransduction that occurs at the integrin-mediated adhesions with the extracellular matrix and the major mechanosensors talin and vinculin. However, the approaches that the cell uses to sense and respond to forces can be applied to other systems, and this therefore provides a general discussion of the force dependence of biomolecule interactions.

Item Type:	Article
Uncontrolled Keywords:	Focal Adhesions, Extracellular Matrix, Animals, Humans, Talin, Vinculin, Integrins, Cell Adhesion, Mechanotransduction, Cellular, Protein Binding, Biophysical Phenomena
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User:	Symplectic Admin
Date Deposited:	13 Mar 2024 10:04
Last Modified:	13 Mar 2024 10:04
DOI:	10.1021/acs.biochem.9b00453
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3179295