Yu, Miao, Le, Shimin 
ORCID: 0000-0003-2359-1897, Ammon, York-Christoph, Goult, Benjamin T ORCID: 0000-0002-3438-2807, Akhmanova, Anna and Yan, Jie ORCID: 0000-0002-8555-7291
(2019)
Force-Dependent Regulation of Talin-KANK1 Complex at Focal Adhesions.
Nano letters, 19 (9).
pp. 5982-5990.
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Abstract
KANK proteins mediate cross-talk between dynamic microtubules and integrin-based adhesions to the extracellular matrix. KANKs interact with the integrin/actin-binding protein talin and with several components of microtubule-stabilizing cortical complexes. Because of actomyosin contractility, the talin-KANK complex is likely under mechanical force, and its mechanical stability is expected to be a critical determinant of KANK recruitment to focal adhesions. Here, we quantified the lifetime of the complex of the talin rod domain R7 and the KN domain of KANK1 under shear-force geometry and found that it can withstand forces for seconds to minutes over a physiological force range up to 10 pN. Complex stability measurements combined with cell biological experiments suggest that shear-force stretching promotes KANK1 localization to the periphery of focal adhesions. These results indicate that the talin-KANK1 complex is mechanically strong, enabling it to support the cross-talk between microtubule and actin cytoskeleton at focal adhesions.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Hela Cells, Focal Adhesions, Extracellular Matrix, Microtubules, Humans, Multiprotein Complexes, Adaptor Proteins, Signal Transducing, Actomyosin, Cytoskeletal Proteins, Talin, Integrins, Cell Adhesion, Mechanotransduction, Cellular, Muscle Contraction, Shear Strength, Mechanical Phenomena, Actin Cytoskeleton |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 13 Mar 2024 10:03 |
| Last Modified: | 13 Mar 2024 10:03 |
| DOI: | 10.1021/acs.nanolett.9b01732 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3179296 |
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