A direct interaction between fascin and microtubules contributes to adhesion dynamics and cell migration.

Villari, Giulia ORCID: 0000-0002-4811-3990, Jayo, Asier ORCID: 0000-0002-9899-8723, Zanet, Jennifer, Fitch, Briana, Serrels, Bryan, Frame, Margaret ORCID: 0000-0001-5882-1942, Stramer, Brian M ORCID: 0000-0001-7034-3269, Goult, Benjamin T ORCID: 0000-0002-3438-2807 and Parsons, Maddy ORCID: 0000-0002-2021-8379 (2015) A direct interaction between fascin and microtubules contributes to adhesion dynamics and cell migration. Journal of cell science, 128 (24). pp. 4601-4614.

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Official URL: http://dx.doi.org/10.1242/jcs.175760

Abstract

Fascin is an actin-binding and bundling protein that is highly upregulated in most epithelial cancers. Fascin promotes cell migration and adhesion dynamics in vitro and tumour cell metastasis in vivo. However, potential non-actin bundling roles for fascin remain unknown. Here, we show for the first time that fascin can directly interact with the microtubule cytoskeleton and that this does not depend upon fascin-actin bundling. Microtubule binding contributes to fascin-dependent control of focal adhesion dynamics and cell migration speed. We also show that fascin forms a complex with focal adhesion kinase (FAK, also known as PTK2) and Src, and that this signalling pathway lies downstream of fascin-microtubule association in the control of adhesion stability. These findings shed light on new non actin-dependent roles for fascin and might have implications for the design of therapies to target fascin in metastatic disease.

Item Type:	Article
Uncontrolled Keywords:	Hela Cells, Microtubules, Humans, Microfilament Proteins, Carrier Proteins, Cell Adhesion, Cell Movement, Focal Adhesion Kinase 1
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User:	Symplectic Admin
Date Deposited:	13 Mar 2024 09:41
Last Modified:	13 Mar 2024 09:41
DOI:	10.1242/jcs.175760
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3179342