Richards, Rosalie, Mason, David, Kelly, Rebecca, Lévy, Raphaël, Bearon, Rachel 
ORCID: 0000-0001-8461-0823 and Sée, Violaine
(2018)
4D imaging and analysis of multicellular tumour spheroid cell migration and invasion.
[Preprint]
Abstract
Studying and characterising tumour cell migration is critical for understanding disease progression and for assessing drug efficacy. Whilst tumour cell migration occurs fundamentally in 3 spatial dimensions (3D), for practical reasons, most migration studies to date have performed analysis in 2D. Here we imaged live multicellular tumour spheroids with lightsheet fluorescence microscopy to determine cellular migration and invasion in 3D over time (4D). We focused on glioblastoma, which are aggressive brain tumours, where cell invasion into the surrounding normal brain remains a major clinical challenge. We developed a workflow for analysing complex 3D cell movement, taking into account migration within the spheroid as well as invasion into the surrounding matrix. This provided metrics characterising cell motion, which we used to evaluate the efficacy of chemother-apeutics on invasion. These rich datasets open avenues for further studies on drug efficacy, microenvironment composition, as well as collective cell migration and metastatic potential.
| Item Type: | Preprint |
|---|---|
| Uncontrolled Keywords: | Rare Diseases, Brain Disorders, Brain Cancer, Cancer, Neurosciences, 2.1 Biological and endogenous factors, 2 Aetiology, Cancer |
| Divisions: | Faculty of Health and Life Sciences Faculty of Science and Engineering > School of Physical Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 19 Mar 2024 11:07 |
| Last Modified: | 19 Mar 2024 11:07 |
| DOI: | 10.1101/443648 |
| Open Access URL: | https://doi.org/10.1101/443648 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3179474 |
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