Thi Hue Kien, Duong 
ORCID: 0000-0002-1573-5697, Edenborough, Kathryn ORCID: 0000-0002-9598-5740, da Silva Goncalves, Daniela, Thuy Vi, Tran, Casagrande, Etiene, Thi Le Duyen, Huynh, Thi Long, Vo, Thi Dui, Le, Thi Tuyet Nhu, Vu, Thi Giang, Nguyen et al (show 11 more authors)
(2023)
Genome evolution of dengue virus serotype 1 under selection by <i>Wolbachia pipientis</i> in <i>Aedes aegypti</i> mosquitoes.
Virus evolution, 9 (1).
vead016-.
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Genome evolution of dengue virus serotype 1 under selection by iWolbachia pipientisi in iAedes aegyptii mosquitoes.pdf - Open Access published version Download (4MB) | Preview |
Abstract
The introgression of antiviral strains of <i>Wolbachia</i> into <i>Aedes aegypti</i> mosquito populations is a public health intervention for the control of dengue. Plausibly, dengue virus (DENV) could evolve to bypass the antiviral effects of <i>Wolbachia</i> and undermine this approach. Here, we established a serial-passage system to investigate the evolution of DENV in <i>Ae. aegypti</i> mosquitoes infected with the <i>w</i>Mel strain of <i>Wolbachia</i>. Using this system, we report on virus genetic outcomes after twenty passages of serotype 1 of DENV (DENV-1). An amino acid substitution, E203K, in the DENV-1 envelope protein was more frequently detected in the consensus sequence of virus populations passaged in <i>w</i>Mel-infected <i>Ae. aegypti</i> than wild-type counterparts. Positive selection at residue 203 was reproducible; it occurred in passaged virus populations from independent DENV-1-infected patients and also in a second, independent experimental system. In wild-type mosquitoes and human cells, the 203K variant was rapidly replaced by the progenitor sequence. These findings provide proof of concept that <i>w</i>Mel-associated selection of virus populations can occur in experimental conditions. Field-based studies are needed to explore whether <i>w</i>Mel imparts selective pressure on DENV evolution in locations where <i>w</i>Mel is established.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Aedes aegypti mosquitoes, biocontrol, dengue virus, virus evolution, wMel Wolbachia |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 18 Mar 2024 15:54 |
| Last Modified: | 18 Mar 2024 15:54 |
| DOI: | 10.1093/ve/vead016 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3179549 |
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