Machine Learning Assisted Handheld Confocal Raman Micro-Spectroscopy for Identification of Clinically Relevant Atopic Eczema Biomarkers.

Dev, Kapil, Ho, Chris Jun Hui, Bi, Renzhe, Yew, Yik Weng, S, Dinish U, Attia, Amalina Binte Ebrahim, Moothanchery, Mohesh ORCID: 0000-0002-6109-3760, Guan, Steven Thng Tien and Olivo, Malini (2022) Machine Learning Assisted Handheld Confocal Raman Micro-Spectroscopy for Identification of Clinically Relevant Atopic Eczema Biomarkers. Sensors (Basel, Switzerland), 22 (13). p. 4674.

Access the full-text of this item by clicking on the Open Access link.

Official URL: http://dx.doi.org/10.3390/s22134674

Abstract

Atopic dermatitis (AD) is a common chronic inflammatory skin dermatosis condition due to skin barrier dysfunction that causes itchy, red, swollen, and cracked skin. Currently, AD severity clinical scores are subjected to intra- and inter-observer differences. There is a need for an objective scoring method that is sensitive to skin barrier differences. The aim of this study was to evaluate the relevant skin chemical biomarkers in AD patients. We used confocal Raman micro-spectroscopy and advanced machine learning methods as means to classify eczema patients and healthy controls with sufficient sensitivity and specificity. Raman spectra at different skin depths were acquired from subjects' lower volar forearm location using an in-house developed handheld confocal Raman micro-spectroscopy system. The Raman spectra corresponding to the skin surface from all the subjects were further analyzed through partial least squares discriminant analysis, a binary classification model allowing the classification between eczema and healthy subjects with a sensitivity and specificity of 0.94 and 0.85, respectively, using stratified K-fold (K = 10) cross-validation. The variable importance in the projection score from the partial least squares discriminant analysis classification model further elucidated the role of important stratum corneum proteins and lipids in distinguishing two subject groups.

Item Type:	Article
Uncontrolled Keywords:	Skin, Humans, Dermatitis, Atopic, Eczema, Spectrum Analysis, Raman, Biomarkers, Machine Learning
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Tech, Infrastructure and Environmental Directorate
Depositing User:	Symplectic Admin
Date Deposited:	26 Mar 2024 11:04
Last Modified:	26 Mar 2024 15:30
DOI:	10.3390/s22134674
Open Access URL:	https://doi.org/10.3390/s22134674
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3179968