Malpartida-Cardenas, Kenny, Miglietta, Luca, Peng, Tianyi, Moniri, Ahmad, Holmes, Alison ORCID: 0000-0001-5554-5743, Georgiou, Pantelis and Rodriguez-Manzano, Jesus
(2022)
Single-channel digital LAMP multiplexing using amplification curve analysis.
Sensors & diagnostics, 1 (3).
pp. 465-468.
Abstract
Loop-mediated isothermal amplification assays are currently limited to one target per reaction in the absence of melting curve analysis, molecular probes or restriction enzyme digestion. Here, we demonstrate multiplexing of five targets in a single fluorescent channel using digital LAMP and the machine learning-based method amplification curve analysis, resulting in a classification accuracy of 91.33% on 54 186 positive amplification events.
Item Type: | Article |
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Uncontrolled Keywords: | Biotechnology |
Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology |
Depositing User: | Symplectic Admin |
Date Deposited: | 09 Apr 2024 09:58 |
Last Modified: | 09 Apr 2024 13:52 |
DOI: | 10.1039/d2sd00038e |
Open Access URL: | https://doi.org/10.1039/D2SD00038E |
Related URLs: | |
URI: | https://livrepository.liverpool.ac.uk/id/eprint/3180210 |