Molecular Staging of Surgical Margins in Oral Squamous Cell Carcinoma Using Promoter Methylation of p16<SUP>INK4A</SUP>, Cytoglobin, E-cadherin, and <i>TMEFF2</i>



Shaw, Richard J ORCID: 0000-0001-7027-8997, Hobkirk, Andrew J, Nikolaidis, George, Woolgar, Julia A, Triantafyllou, Asterios, Brown, James S, Liloglou, Triantafillos ORCID: 0000-0003-0460-1404 and Risk, Janet M ORCID: 0000-0002-8770-7783
(2013) Molecular Staging of Surgical Margins in Oral Squamous Cell Carcinoma Using Promoter Methylation of p16<SUP>INK4A</SUP>, Cytoglobin, E-cadherin, and <i>TMEFF2</i>. ANNALS OF SURGICAL ONCOLOGY, 20 (8). pp. 2796-2802.

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Abstract

<h4>Background</h4>Local recurrence in oral squamous cell carcinoma (OSCC) despite clear surgical margins may indicate the presence of residual, sub-microscopic disease. Molecular assessment of surgical margins may provide a greater prognostic sensitivity compared to histopathology. We aimed to determine whether promoter methylation in deep and mucosal resection margins can predict recurrence in OSCC.<h4>Methods</h4>Forty-eight consecutive OSCC cases were recruited and a 5 mm(3) tumor sample plus 5 deep and 5 mucosal margin samples were snap frozen. Clinical, pathological, adjuvant therapy, and outcome data were recorded. Tumors were informative if >5 % promoter methylation was found for ≥1 of 4 genes using qMSP. Margins were declared molecularly positive if >1 % promoter methylation was found in any margin.<h4>Results</h4>Thirty (63 %) of 48 cases were methylation informative. Mucosal margin samples were largely positive for methylation (26 of 30, 87 %), indicating the presence of field cancerization. Methylation at ≥1 gene promoters in ≥1 deep margin correlated with the presence of close/involved mucosal margins (P = 0.027) and increased pT status (P = 0.027) but not the status of deep margins, recurrence, or survival.<h4>Conclusions</h4>The current gene panel did not add prognostic information to histopathological reporting of resection margins. Future efforts should concentrate on improving gene selection, informativity, and assay performance in the patient group with intermediate indications for adjuvant therapy.

Item Type: Article
Additional Information: Issue date: 1st August 2013. Available online 31 October 2012.
Uncontrolled Keywords: Humans, Carcinoma, Squamous Cell, Mouth Neoplasms, Neoplasm Recurrence, Local, Neoplasm, Residual, Globins, Cadherins, Membrane Proteins, Neoplasm Proteins, DNA Methylation, Genes, p16, Aged, Middle Aged, Female, Male, Promoter Regions, Genetic, Kaplan-Meier Estimate, Cytoglobin
Subjects: ?? RK ??
Divisions: Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User: Symplectic Admin
Date Deposited: 20 Nov 2012 16:28
Last Modified: 18 Oct 2023 09:55
DOI: 10.1245/s10434-012-2713-8
Publisher's Statement : Originally published in Annals of Surgical Oncology. The final publication is available at The final publication is available at Springer via http://dx.doi.org/10.1245/s10434-012-2713-8.
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/7701

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  • Molecular Staging of Surgical Margins in Oral Squamous Cell Carcinoma Using Promoter Methylation of p16<SUP>INK4A</SUP>, Cytoglobin, E-cadherin, and <i>TMEFF2</i>. (deposited 20 Nov 2012 16:28) [Currently Displayed]