Molecular monitoring of the Leu-164 mutation of dihydrofolate reductase in a highly sulfadoxine/pyrimethamine-resistant area in Africa.


Ochong, Edwin, Nzila, Alexis, Kimani, Serah, Kokwaro, Gilbert, Mutabingwa, Theonest, Watkins, William and Marsh, Kevin (2003) Molecular monitoring of the Leu-164 mutation of dihydrofolate reductase in a highly sulfadoxine/pyrimethamine-resistant area in Africa. Malaria journal, 2 (1). 46-.

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Abstract

The selection of point mutation at codon 164 (from isoleucine to leucine) of the dihydrofolate reductase (DHFR) enzyme in Plasmodium falciparum is associated with high sulfadoxine /pyrimethamine (SP) resistance. Using the yeast expression system that allows the detection of dhfr allele present at low level, the presence of this mutation had previously been reported between 1998-1999 in Muheza, Tanzania, an area of high SP resistance. Eighty five P. falciparum isolates, obtained from the same area between 2002 and 2003, were analysed for the presence of Leu-164 mutation, using standard protocol based on PCR-RFLP. None of the isolates had the Leu-164 mutation.

Item Type: Article
Additional Information: Published: 15 December 2003. 3 pages (page numbers not for citation purposes).
Uncontrolled Keywords: PYRIMETHAMINE-SULFADOXINE RESISTANCE, PLASMODIUM-FALCIPARUM, CHLORPROGUANIL-DAPSONE, MALARIA, PARASITES, LAPDAP
Subjects: ?? R1 ??
Divisions: Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User: Symplectic Admin
Date Deposited: 07 Jul 2008 09:23
Last Modified: 16 Mar 2024 13:17
DOI: 10.1186/1475-2875-2-46
Publisher's Statement : © 2003 Ochong et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL. This article is available from: http://www.malariajournal.com/content/2/1/46
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URI: https://livrepository.liverpool.ac.uk/id/eprint/783
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