<i>Sprouty2</i> mediated tuning of signalling is essential for somite myogenesis

Abu-Elmagd, Muhammad, Whysall, Katarzyna Goljanek, Wheeler, Grant and Muensterberg, Andrea (2015) <i>Sprouty2</i> mediated tuning of signalling is essential for somite myogenesis. BMC MEDICAL GENOMICS, 8 (Suppl ). S8-.

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Abstract

<h4>Background</h4>Negative regulators of signal transduction cascades play critical roles in controlling different aspects of normal embryonic development. Sprouty2 (Spry2) negatively regulates receptor tyrosine kinases (RTK) and FGF signalling and is important in differentiation, cell migration and proliferation. In vertebrate embryos, Spry2 is expressed in paraxial mesoderm and in forming somites. Expression is maintained in the myotome until late stages of somite differentiation. However, its role and mode of action during somite myogenesis is still unclear.<h4>Results</h4>Here, we analysed chick Spry2 expression and showed that it overlaps with that of myogenic regulatory factors MyoD and Mgn. Targeted mis-expression of Spry2 led to inhibition of myogenesis, whilst its C-terminal domain led to an increased number of myogenic cells by stimulating cell proliferation.<h4>Conclusions</h4>Spry2 is expressed in somite myotomes and its expression overlaps with myogenic regulatory factors. Overexpression and dominant-negative interference showed that Spry2 plays a crucial role in regulating chick myogenesis by fine tuning of FGF signaling through a negative feedback loop. We also propose that mir-23, mir-27 and mir-128 could be part of the negative feedback loop mechanism. Our analysis is the first to shed some light on in vivo Spry2 function during chick somite myogenesis.

Item Type:	Article
Uncontrolled Keywords:	Somites, Animals, Chickens, Fibroblast Growth Factor 2, Intracellular Signaling Peptides and Proteins, MicroRNAs, Signal Transduction, Mitosis, Gene Expression Regulation, Developmental, Muscle Development, Feedback, Physiological
Subjects:	?? QH301 ??
Depositing User:	Symplectic Admin
Date Deposited:	31 Mar 2015 16:04
Last Modified:	11 Oct 2023 22:43
DOI:	10.1186/1755-8794-8-S1-S8
Publisher's Statement :	© 2015 Abu-Elmagd et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/2009403