Introducing Discrete Frequency Infrared Technology for High-Throughput Biofluid Screening



Hughes, Caryn, Clemens, Graeme, Bird, Benjamin, Dawson, Timothy, Ashton, Katherine M, Jenkinson, Michael D ORCID: 0000-0003-4587-2139, Brodbelt, Andrew, Weida, Miles, Fotheringham, Edeline, Barre, Matthew
et al (show 2 more authors) (2016) Introducing Discrete Frequency Infrared Technology for High-Throughput Biofluid Screening. SCIENTIFIC REPORTS, 6 (1). 20173-.

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Abstract

Accurate early diagnosis is critical to patient survival, management and quality of life. Biofluids are key to early diagnosis due to their ease of collection and intimate involvement in human function. Large-scale mid-IR imaging of dried fluid deposits offers a high-throughput molecular analysis paradigm for the biomedical laboratory. The exciting advent of tuneable quantum cascade lasers allows for the collection of discrete frequency infrared data enabling clinically relevant timescales. By scanning targeted frequencies spectral quality, reproducibility and diagnostic potential can be maintained while significantly reducing acquisition time and processing requirements, sampling 16 serum spots with 0.6, 5.1 and 15% relative standard deviation (RSD) for 199, 14 and 9 discrete frequencies respectively. We use this reproducible methodology to show proof of concept rapid diagnostics; 40 unique dried liquid biopsies from brain, breast, lung and skin cancer patients were classified in 2.4 cumulative seconds against 10 non-cancer controls with accuracies of up to 90%.

Item Type: Article
Additional Information: Cite as; Hughes, C. et al. Introducing Discrete Frequency Infrared Technology for HighTroughput Biofuid Screening. Sci. Rep. 6, 20173; doi: 10.1038/srep20173 (2016).
Uncontrolled Keywords: Body Fluids, Humans, Breast Neoplasms, Brain Neoplasms, Skin Neoplasms, Microscopy, Confocal, Biopsy, Spectrophotometry, Infrared, Reproducibility of Results, Automation, Female, Lasers, Semiconductor, Dried Blood Spot Testing
Depositing User: Symplectic Admin
Date Deposited: 05 Feb 2016 17:19
Last Modified: 15 Dec 2022 15:05
DOI: 10.1038/srep20173
Publisher's Statement : Tis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
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URI: https://livrepository.liverpool.ac.uk/id/eprint/2050208