Sensitive Analysis of Protein Adsorption to Colloidal Gold by Differential Centrifugal Sedimentation

Davidson, Adam M, Brust, Mathias ORCID: 0000-0001-6301-7123, Cooper, David L ORCID: 0000-0003-0639-0794 and Volk, Martin ORCID: 0000-0003-3555-8584 (2017) Sensitive Analysis of Protein Adsorption to Colloidal Gold by Differential Centrifugal Sedimentation. ANALYTICAL CHEMISTRY, 89 (12). pp. 6807-6814.

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Abstract

It is demonstrated that the adsorption of bovine serum albumin (BSA) to aqueous gold colloids can be quantified with molecular resolution by differential centrifugal sedimentation (DCS). This method separates colloidal particles of comparable density by mass. When proteins adsorb to the nanoparticles, both their mass and their effective density change, which strongly affects the sedimentation time. A straightforward analysis allows quantification of the adsorbed layer. Most importantly, unlike many other methods, DCS can be used to detect chemisorbed proteins ("hard corona") as well as physisorbed proteins ("soft corona"). The results for BSA on gold colloid nanoparticles can be modeled in terms of Langmuir-type adsorption isotherms (Hill model). The effects of surface modification with small thiol-PEG ligands on protein adsorption are also demonstrated.

Item Type:	Article
Uncontrolled Keywords:	Animals, Cattle, Gold Colloid, Polyethylene Glycols, Sulfhydryl Compounds, Serum Albumin, Bovine, Ligands, Ultracentrifugation, Adsorption, Nanoparticles
Depositing User:	Symplectic Admin
Date Deposited:	18 May 2017 06:22
Last Modified:	19 Jan 2023 07:04
DOI:	10.1021/acs.analchem.7b01229
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3007519