Oxidative stress alters mitochondrial bioenergetics and modifies pancreatic cell death independently of cyclophilin D, resulting in an apoptosis-to-necrosis shift

Armstrong, Jane A, Cash, Nicole J, Ouyang, Yulin, Morton, Jack C, Chvanov, Michael, Latawiec, Diane, Awais, Muhammad, Tepikin, Alexei V, Sutton, Robert ORCID: 0000-0001-6600-562X and Criddle, David N ORCID: 0000-0003-2952-8450 (2018) Oxidative stress alters mitochondrial bioenergetics and modifies pancreatic cell death independently of cyclophilin D, resulting in an apoptosis-to-necrosis shift. JOURNAL OF BIOLOGICAL CHEMISTRY, 293 (21). pp. 8032-8047.

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Official URL: https://www.jbc.org/content/early/2018/04/06/jbc.R...

Abstract

Mitochondrial dysfunction lies at the core of acute pancreatitis (AP). Diverse AP stimuli induce Ca2+-dependent formation of the mitochondrial permeability transition pore (MPTP), a solute channel modulated by cyclophilin D (CypD), whose formation causes ATP depletion and necrosis. Oxidative stress reportedly triggers MPTP formation and is elevated in clinical AP, but how reactive oxygen species influence cell death is unclear. Here, we assessed potential MPTP involvement in oxidant-induced effects on pancreatic acinar cell bioenergetics and fate. H2O2 application promoted acinar cell apoptosis at low concentrations (1-10 μM), whereas higher levels (0.5-1 mM) elicited rapid necrosis. H2O2 also decreased the mitochondrial NADH/FAD+ redox ratio and ΔΨm in a concentration-dependent manner (10 μM-1 mM H2O2), with maximal effects at 500 μM H2O2. H2O2 decreased the basal O2 consumption rate of acinar cells, with no alteration of ATP turnover at < 50 μM H2O2. However, higher H2O2 levels (≥ 50μM) diminished spare respiratory capacity and ATP turnover, and bioenergetic collapse, ATP depletion, and cell death ensued. Menadione exerted detrimental bioenergetic effects similar to those of H2O2, which were inhibited by the antioxidant N-acetylcysteine. Oxidant-induced bioenergetic changes, loss of ΔΨm, and cell death were not ameliorated by genetic deletion of CypD or by its acute inhibition with cyclosporine A. These results indicate that oxidative stress alters mitochondrial bioenergetics and modifies pancreatic acinar cell death. A shift from apoptosis to necrosis appears to be associated with decreased mitochondrial spare respiratory capacity and ATP production, effects that are independent of CypD-sensitive MPTP formation.

Item Type:	Article
Uncontrolled Keywords:	pancreas, oxidative stress, mitochondrial permeability transition (MPT), cyclophilin D, bioenergetics, reactive oxygen species (ROS), apoptosis, necrosis (necrotic death), antioxidant, Acute Pancreatitis, Seahorse
Depositing User:	Symplectic Admin
Date Deposited:	15 Jun 2018 15:00
Last Modified:	19 Jan 2023 06:35
DOI:	10.1074/jbc.RA118.003200
Open Access URL:	http://dx.doi.org/10.1074/jbc.RA118.003200
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3020396