Judice, Carla Cristina, Tan, Jeslin JL, Parise, Pierina Lorencini, Kam, Yiu-Wing, Milanez, Guilherme Paier, Leite, Juliana Almeida, Caserta, Leonardo Cardia, Arns, Clarice Weis, Resende, Mariangela Ribeiro, Angerami, Rodrigo et al (show 7 more authors)
(2018)
Efficient detection of Zika virus RNA in patients' blood from the 2016 outbreak in Campinas, Brazil.
SCIENTIFIC REPORTS, 8 (1).
4012-.
Abstract
Infection with Zika virus (ZIKV), a mosquito-borne flavivirus has been casually linked with increased congenital microcephaly in Brazil from 2015 through 2016. Sensitive and specific diagnosis of patients with Zika fever (ZIKF) remains critical for patient management. We developed a ZIKV NS5 qRT-PCR assay by combining primers described by Balm et al. and a new Taqman probe. The assay was evaluated and compared with another assay described by Lanciotti et al. (ZIKV 1107) using 51 blood and 42 urine samples from 54 suspected ZIKV patients. ZIKV NS5 performed better in terms of sensitivity with more samples detected as ZIKV-positive (n = 37) than ZIKV 1107 (n = 34) for urine, and ZIKV-positive (n = 29) than ZIKV 1107 (n = 26) for blood. Both assays displayed good overall agreement for urine (κappa = 0.770) and blood (κappa = 0.825) samples. Improved availability of validated diagnostic tests, such ZIKV NS5 qRT-PCR, will be critical to ensure adequate and accurate ZIKV diagnosis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Zika-Unicamp Network, Humans, RNA, Viral, Sensitivity and Specificity, Disease Outbreaks, Adult, Middle Aged, Brazil, Female, Male, Young Adult, Limit of Detection, Zika Virus, Zika Virus Infection |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 07 Feb 2019 11:32 |
| Last Modified: | 19 Jan 2023 01:05 |
| DOI: | 10.1038/s41598-018-22159-2 |
| Open Access URL: | https://doi.org/10.1038/s41598-018-22159-2 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3032429 |
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