Roles of RbcX in carboxysome biosynthesis in the cyanobacterium Synechococcus elongatus PCC7942

Huang, fang, Vasieva, olga, Sun, Yaqi, Faulkner, Matthew ORCID: 0000-0002-0578-388X, Dykes, Gregory ORCID: 0000-0002-0626-9487, Zhao, Ziyu and Liu, L ORCID: 0000-0002-8884-4819 (2019) Roles of RbcX in carboxysome biosynthesis in the cyanobacterium Synechococcus elongatus PCC7942. Plant Physiology, 179 (1). pp. 184-194.

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Abstract

Rubisco is the essential enzyme mediating the fixation of atmospheric CO2 during photosynthesis. In cyanobacteria, Rubisco enzymes are densely packed and encapsulated in a specialized organelle known as the carboxysome. Well-defined Rubisco assembly and carboxysome formation are pivotal for efficient CO2 fixation. Numerous chaperone proteins, including RbcX, are essential for proper protein folding and Rubisco assembly. In this study, we investigated the in vivo function of RbcX in the cyanobacterium Synechococcus elongatus PCC 7942 (Syn7942) using molecular, biochemical, and live-cell fluorescence imaging approaches. Our results show that genetic deletion of the rbcX gene affects Rubisco abundance, as well as carboxysome formation and spatial distribution. Moreover, RbcX appears as one component of the carboxysome and shows a dynamic interaction with Rubisco enzymes. These in vivo observations provide insight into the role of RbcX from Syn7942 in mediating carboxysome assembly. Understanding the molecular mechanism underlying Rubisco assembly and carboxysome biogenesis will provide essential information required for engineering functional CO2-fixing complexes in heterogeneous organisms, especially plants, with the aim of boosting photosynthesis and agricultural productivity.

Item Type:	Article
Uncontrolled Keywords:	Organelles, Synechococcus, Carbon Dioxide, Bacterial Proteins, Molecular Chaperones, Computational Biology, Phylogeny, Photosynthesis
Depositing User:	Symplectic Admin
Date Deposited:	12 Feb 2019 12:43
Last Modified:	19 Jan 2023 01:03
DOI:	10.1104/pp.18.01217
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3032729

Available Versions of this Item

• Roles of RbcX in carboxysome biosynthesis in the cyanobacterium Synechococcus elongatus PCC7942. (deposited 24 Oct 2018 11:10)
  • Roles of RbcX in Carboxysome Biosynthesis in the Cyanobacterium Synechococcus elongatus PCC7942. (deposited 05 Nov 2018 09:12)
    • Roles of RbcX in carboxysome biosynthesis in the cyanobacterium Synechococcus elongatus PCC7942. (deposited 12 Feb 2019 12:43) [Currently Displayed]