Jin, Liting, Qu, Ying, Gomez, Liliana J, Chung, Stacey, Han, Bingchen, Gao, Bowen, Yue, Yong, Gong, Yiping, Liu, Xuefeng, Amersi, Farin et al (show 3 more authors)
(2017)
Characterization of primary human mammary epithelial cells isolated and propagated by conditional reprogrammed cell culture.
Oncotarget, 9 (14).
pp. 11503-11514.
Abstract
<h4>Purpose</h4>Conditional reprogramming methods allow for the inexhaustible <i>in vitro</i> proliferation of primary epithelial cells from human tissue specimens. This methodology has the potential to enhance the utility of primary cell culture as a model for mammary gland research. However, few studies have systematically characterized this method in generating <i>in vitro</i> normal human mammary epithelial cell models.<h4>Results</h4>We show that cells derived from fresh normal breast tissues can be propagated and exhibit heterogeneous morphologic features. The cultures are composed of CK18, desmoglein 3, and CK19-positive luminal cells and vimentin, p63, and CK14-positive myoepithelial cells, suggesting the maintenance of <i>in vivo</i> heterogeneity. In addition, the cultures contain subpopulations with different CD49f and EpCAM expression profiles. When grown in 3D conditions, cells self-organize into distinct structures that express either luminal or basal cell markers. Among these structures, CK8-positive cells enclosing a lumen are capable of differentiation into milk-producing cells in the presence of lactogenic stimulus. Furthermore, our short-term cultures retain the expression of ERα, as well as its ability to respond to estrogen stimulation.<h4>Materials and methods</h4>We have investigated conditionally reprogrammed normal epithelial cells in terms of cell type heterogeneity, cellular marker expression, and structural arrangement in two-dimensional (2D) and three-dimensional (3D) systems.<h4>Conclusions</h4>The conditional reprogramming methodology allows generation of a heterogeneous culture from normal human mammary tissue <i>in vitro</i>. We believe that this cell culture model will provide a valuable tool to study mammary cell function and malignant transformation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | conditional reprogramming, estrogen receptor, luminal cells, mammary epithelial cells, myoepithelial cells |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 30 Apr 2019 10:01 |
| Last Modified: | 16 Mar 2023 22:11 |
| DOI: | 10.18632/oncotarget.23817 |
| Open Access URL: | https://doi.org/10.18632/oncotarget.23817 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3039038 |
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