Parkinson's Disease: A Systemic Inflammatory Disease Accompanied by Bacterial Inflammagens.

Adams, Büin, Nunes, J Massimo ORCID: 0000-0003-2584-021X, Page, Martin J ORCID: 0000-0002-2479-9182, Roberts, Timothy ORCID: 0000-0002-1464-0151, Carr, Jonathan ORCID: 0000-0002-7677-426X, Nell, Theo A ORCID: 0000-0003-3905-827X, Kell, Douglas B and Pretorius, Etheresia (2019) Parkinson's Disease: A Systemic Inflammatory Disease Accompanied by Bacterial Inflammagens. Frontiers in aging neuroscience, 11. 210-.

Access the full-text of this item by clicking on the Open Access link.

	Text Parkinsons Disease A Systemic Inflammatory Disease Accompanied by Bacterial Inflammagens.pdf - Published version Download (4MB) | Preview
	Text Parkinsons Disease A Systemic Inflammatory Disease Accompanied by Bacterial Inflammagens.pdf - Published version Download (4MB) | Preview

Abstract

Parkinson's disease (PD) is a well-known neurodegenerative disease with a strong association established with systemic inflammation. Recently, the role of the gingipain protease group from <i>Porphyromonas gingivalis</i> was implicated in Alzheimer's disease and here we present evidence, using a fluorescent antibody to detect gingipain R1 (RgpA), of its presence in a PD population. To further elucidate the action of this gingipain, as well as the action of the lipopolysaccharide (LPS) from <i>P. gingivalis</i>, low concentrations of recombinant RgpA and LPS were added to purified fluorescent fibrinogen. We also substantiate previous findings regarding PD by emphasizing the presence of systemic inflammation via multiplex cytokine analysis, and demonstrate hypercoagulation using thromboelastography (TEG), confocal and electron microscopy. Biomarker analysis confirmed significantly increased levels of circulating proinflammatory cytokines. In our PD and control blood analysis, our results show increased hypercoagulation, the presence of amyloid formation in plasma, and profound ultrastructural changes to platelets. Our laboratory analysis of purified fibrinogen with added RgpA, and/or LPS, showed preliminary data with regards to the actions of the protease and the bacterial membrane inflammagen on plasma proteins, to better understand the nature of established PD.

Item Type:	Article
Depositing User:	Symplectic Admin
Date Deposited:	29 Nov 2019 13:10
Last Modified:	27 Jan 2024 03:01
DOI:	10.3389/fnagi.2019.00210
Open Access URL:	https://doi.org/10.3389/fnagi.2019.00210
Related URLs:	Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3064044