Proteomic and transcriptomic analysis of the protozoan parasite Neospora caninum

Vermont, Sarah J (2012) Proteomic and transcriptomic analysis of the protozoan parasite Neospora caninum. Doctor of Philosophy thesis, University of Liverpool.

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Abstract

Neospora caninum is an economically significant parasitic protozoan causing the disease neosporosis in cattle and dogs. Although a close relative of the zoonotic apicomplexan Toxoplasma gondii, the two organisms exhibit differing host ranges and infection dynamics. T. gondii is a model organism that has been much studied, and a great deal is known about the genes and proteins involved when it invades a host cell. This thesis explores protein expression in the proliferative and invasive tachyzoite stage of N. caninum, in particular the expression of proteins pertaining to the apical complex of organelles; those responsible for entry and establishment within a host cell. Almost 20 % of the predicted proteome has been identified by this analysis to be expressed in the tachyzoite stage, with approximately 50 % of the predicted repertoire of apical proteins being detected. The discovery of differences between these two parasites’ highly syntenic genomes could lead to a better understanding of the process by which T. gondii is able to cause disease in humans, while N. caninum has not been observed to do so. One finding of the recent genome sequencing and annotation project in N. caninum was that a key T. gondii ... (continues)

Item Type:	Thesis (Doctor of Philosophy)
Additional Information:	Date: 2012-09 (completed)
Uncontrolled Keywords:	Neospora caninum, Apicomplexa, Proteomics, Transcriptomics, parasite, Toxoplasma gondii, protozoan, rhoptry, microneme, dense granule
Divisions:	Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences
Depositing User:	Symplectic Admin
Date Deposited:	04 Sep 2013 11:19
Last Modified:	31 Aug 2024 13:37
DOI:	10.17638/00011637
Supervisors:	Wastling, Jonathan Jones, Andy
URI:	https://livrepository.liverpool.ac.uk/id/eprint/11637